Partial purification and characterization of RNase P from human peripheral lymphocytes
| Autor: | Alexandra Monastirli, Anastassios Vourekas, Dionysios Tsambaos, Chrisavgi Toumpeki, Denis Drainas, Vasiliki Stamatopoulou, Eleftheria Vryzaki |
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| Rok vydání: | 2009 |
| Předmět: |
Chromatography
Dose-Response Relationship Drug biology RNase P Endoribonuclease Ribozyme Dermatology Transfection Biochemistry Molecular biology Ribonuclease P Cell biology Enzyme Activation RNase MRP Transfer RNA biology.protein Autoradiography Humans Lymphocytes Enzyme Inhibitors RNase H Molecular Biology Framycetin Ribonucleoprotein |
| Zdroj: | Experimental Dermatology. 18:130-133 |
| ISSN: | 1600-0625 0906-6705 |
| DOI: | 10.1111/j.1600-0625.2008.00772.x |
| Popis: | Ribonuclease P (RNase P) is ubiquitous and essential Mg(2+)-dependent endoribonuclease that catalyzes the 5'-maturation of transfer RNAs. RNase P and the ribosome are so far the only ribozymes known to be conserved in all kingdoms of life. Eukaryotic RNase P activity has been detected in nuclei, mitochondria and chloroplasts and demonstrates great variability in sequence and subunit composition. In the last few years we have developed methodologies and pursued projects addressing the occurrence, distribution and the potential physiological role of RNase P in human epidermal keratinocytes. In view of the vital importance of lymphocytes for an effective immune system and their successful application after transfection with RNase P-associated external guide sequences in gene therapy, we concerned ourselves with the isolation and characterization of RNase P of peripheral human lymphocytes. We developed a method described herein, that will enable the study of the possible involvement of this ribozyme in the pathogenetic mechanisms of diverse autoimmune, inflammatory and neoplastic cutaneous disorders and may facilitate the further development of RNase P-based technology for gene therapy of infectious and neoplastic dermatoses. |
| Databáze: | OpenAIRE |
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