A Rapid, Direct, Quantitative, and Label-Free Detector of Cardiac Biomarker Troponin T Using Near-Infrared Fluorescent Single-Walled Carbon Nanotube Sensors
| Autor: | Nigel F. Reuel, Michael S. Strano, Daniel A. Heller, Paul W. Barone, S. Shimizu, Flor De La Cruz, Jingqing Zhang, Sebastian Kruss, Andrew J. Hilmer, Zeke Schmois |
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| Rok vydání: | 2013 |
| Předmět: |
Materials science
Myocardial Infarction Biomedical Engineering Pharmaceutical Science Nanotechnology Biosensing Techniques Microscopy Atomic Force Biomaterials chemistry.chemical_compound Troponin T Troponin complex Troponin I medicine Humans Myocardial infarction Detection limit Chitosan Spectroscopy Near-Infrared Chromatography biology Nanotubes Carbon Nitrilotriacetic acid medicine.disease Fluorescence Troponin chemistry biology.protein Biomarkers |
| Zdroj: | Advanced Healthcare Materials. 3:412-423 |
| ISSN: | 2192-2640 |
| DOI: | 10.1002/adhm.201300033 |
| Popis: | Patients with chest pain account for 10% of US emergency room visits according to data from the Center for Disease Control and Prevention (2013). For triage of these patients, cardiac biomarkers troponin I and T are endorsed as standard indicators for acute myocardial infarction (AMI, or heart attack). Thus, there is significant interest in developing a rapid, point-of-care (POC) device for troponin detection. In this work, a rapid, quantitative, and label-free assay, which is specific for cardiac troponin T (cTnT) detection, using fluorescent single-walled carbon nanotubes (SWCNTs), is demonstrated. Chitosan-wrapped carbon nanotubes are cross-linked to form a thin gel that is further functionalized with nitrilotriacetic acid (NTA) moieties. Upon chelation of Ni(2+) , the Ni(2+) -NTA group binds to a hexa-histidine-modified troponin antibody, which specifically recognizes the target protein, troponin T. As the troponin T binds to the antibody, the local environment of the sensor changes, allowing direct troponin detection through intensity changes in SWCNT bandgap fluorescence. This platform represents the first near-infrared SWCNT sensor array for cTnT detection. Detection can be completed within 5 min, demonstrating a linear response to cTnT concentration and an experimental detection limit of 100 ng mL(-1) (2.5 nm). This platform provides a promising new tool for POC AMI detection in the future. Moreover, the work presents two new methods of quantifying the number of amines and carboxylic groups, respectively, in a carbon hydrogel matrices. |
| Databáze: | OpenAIRE |
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