Baicalein induced in vitro apoptosis undergo caspases activity in human promyelocytic leukemia HL-60 cells
| Autor: | Yu Ching Li, Y.S. Tyan, Hui-Chun Kuo, Te Chun Hsia, W. C. Chang, Jing-Gung Chung |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Programmed cell death
Cell Survival Apoptosis HL-60 Cells DNA Fragmentation Toxicology Antioxidants Amino Acid Chloromethyl Ketones chemistry.chemical_compound medicine Humans Viability assay Enzyme Inhibitors Caspase Flavonoids biology Cell Cycle Antibodies Monoclonal General Medicine Trypan Blue medicine.disease Flow Cytometry Molecular biology Caspase Inhibitors In vitro Baicalein Leukemia Biochemistry chemistry Caspases Flavanones biology.protein DNA fragmentation Indicators and Reagents Poly(ADP-ribose) Polymerases Food Science |
| Zdroj: | Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association. 42(1) |
| ISSN: | 0278-6915 |
| Popis: | In this study, we evaluated the potential apoptosis effects of baicalein on human promyelocytic leukemia HL-60 cells in vitro. Apoptosis induction, cell viability, morphology and caspase-3 activity were then performed to determine by flow cytometric assay, DNA gel electrophoresis, anti-ADP-ribose stain and determination of caspase-3 activity. There is a significant difference in cell death of HL-60 cells that was detected between baicalein-treated and untreated groups. Furthermore, there was a further significant increase in apoptosis induction when cells were treated with baicalein compared to without baicalein treated groups. Flow cytometric assays and DNA fragmentation gel electrophoresis also confirmed baicalein induced apoptosis in HL-60 cells. Baicalein also promoted caspase-3 activity then leading to cleavage of poly-ADP-ribose polymerase finally leading to DNA fragmentation occurrence. Furthermore, the baicalein-induced apoptosis was markedly blocked by the broad-spectrum caspase inhibitor, z-VAD-fmk. Taken together, these results suggest that treatment of human leukemia HL-60 cells with baicalein induced apoptosis through activation of caspase-3 activity. |
| Databáze: | OpenAIRE |
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