Validation of a Lysis Buffer Containing 4 M Guanidinium Thiocyanate (GITC)/ Triton X-100 for Extraction of SARS-CoV-2 RNA for COVID-19 Testing: Comparison of Formulated Lysis Buffers Containing 4 to 6 M GITC, Roche External Lysis Buffer and Qiagen RTL Lysis Buffer

Autor:	Paula M. O'Connor, John McSharry, Brigid Lucey, Sarah P. Hudson, Martina F. Scallan, Isabelle O'Callaghan, Conor P. Horgan, Paul D. Cotter, Humphrey A. Moynihan, Edel Durack, Catherine Dempsey
Jazyk:	angličtina
Rok vydání:	2020
Předmět:	chemistry.chemical_compound Guanidinium thiocyanate Lysis Chromatography chemistry Coronavirus disease 2019 (COVID-19) Extraction (chemistry) Lysis buffer Triton X-100 RNA Viral rna
DOI:	10.1101/2020.04.05.026435
Popis:	The COVID-19 pandemic has resulted in increased need for diagnostic testing using reverse transcriptase real-time PCR (RT-PCR). An exponential increase in demand has resulted in a shortage of numerous reagents in particular those associated with the lysis buffer required to extract the viral RNA. Herein, we describe a rapid collective effort by hospital laboratory scientists, academic researchers and the biopharma industry to generate a validated lysis buffer. We have formulated a 4M Guanidinium thiocyanate (GITC)/ Triton X-100 Lysis buffer which provides comparable results with the recommended reagents. This buffer will ease the burden on hospital labs in their heroic efforts to diagnose a large population of patients.
Databáze:	OpenAIRE
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