Immunological and bacteriological shifts associated with a flagellin-hyperproducing Salmonella Enteritidis mutant in chickens
| Autor: | Fernanda de Oliveira Barbosa, Mauro M.S. Saraiva, Marcela da Silva Rubio, Lucas Bocchini Rodrigues Alves, Adriana Maria de Almeida, Celso José Bruno de Oliveira, Angelo Berchieri Junior, Andrei Itajahy Secundo de Souza, Valdinete Pereira Benevides, Oliveiro C. Freitas Neto, John Elmerdahl Olsen |
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| Přispěvatelé: | Universidade Estadual Paulista (Unesp), Universidade Federal de Minas Gerais (UFMG), Universidade Federal da Paraíba (UFPB), Univ Copenhagen |
| Rok vydání: | 2020 |
| Předmět: |
Salmonella
Salmonellosis Salmonella enteritidis medicine.disease_cause Microbiology Poultry Deletion 03 medical and health sciences Immune system Bacterial Proteins Media Technology medicine Animals Poultry Diseases 030304 developmental biology 0303 health sciences Salmonella Infections Animal biology 030306 microbiology Immunogenicity Veterinary Microbiology - Research Paper Mutant Wild type biology.organism_classification Salmonella enterica Flagella biology.protein Chickens Bacteria Flagellin |
| Zdroj: | Braz J Microbiol Web of Science Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
| ISSN: | 1678-4405 |
| Popis: | Made available in DSpace on 2021-06-25T12:24:03Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-11-04 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Salmonella Enteritidis causes infections in humans and animals which are often associated with extensive gut colonization and bacterial shedding in faeces. The natural presence of flagella in Salmonella enterica has been shown to be enough to induce pro-inflammatory responses in the gut, resulting in recruitment of polymorphonuclear cells, gut inflammation and, consequently, reducing the severity of systemic infection in chickens. On the other hand, the absence of flagellin in some Salmonella strains favours systemic infection as a result of the poor intestinal inflammatory responses elicited. The hypothesis that higher production of flagellin by certain Salmonella enterica strains could lead to an even more immunogenic and less pathogenic strain for chickens was here investigated. In the present study, a Salmonella Enteritidis mutant strain harbouring deletions in clpP and fliD genes (SE Delta clpPfliD), which lead to overexpression of flagellin, was generated, and its immunogenicity and pathogenicity were comparatively assessed to the wild type in chickens. Our results showed that SE Delta clpPfliD elicited more intense immune responses in the gut during early stages of infection than the wild type did, and that this correlated with earlier intestinal and systemic clearance of the bacterium. State Univ Sao Paulo, Sch Agr & Veterinarian Sci FCAV, Dept Vet Pathol, Jaboticabal, Brazil Fed Univ Minas Gerais UFMG, Vet Sch, Dept Prevent Vet Med, Av Antonio Carlos 6627, BR-31270901 Belo Horizonte, MG, Brazil Fed Univ Paraiba CCA UFPB, Ctr Agr Sci, Dept Anim Sci, Areia, PB, Brazil Univ Copenhagen, Dept Vet & Anim Sci, Stigbojlen 4, DK-1870 Frederiksberg C, Denmark State Univ Sao Paulo, Sch Agr & Veterinarian Sci FCAV, Dept Vet Pathol, Jaboticabal, Brazil FAPESP: 2018/04883-8 FAPESP: 2016/10369-0 |
| Databáze: | OpenAIRE |
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