Cloning and Expression of a Subfamily 1.4 Lipase from Bacillus licheniformis IBRL-CHS2
| Autor: | Rashidah Abdul Rahim, K. Sudesh Kumar, Nidyaletchmy Subba Reddy, Darah Ibrahim |
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| Rok vydání: | 2016 |
| Předmět: |
Subfamily
02 engineering and technology 01 natural sciences General Biochemistry Genetics and Molecular Biology DNA sequencing law.invention law Bacillus licheniformis Lipase Gene Cloning Expression vector biology Chemistry 010401 analytical chemistry General Medicine 021001 nanoscience & nanotechnology biology.organism_classification Biotechnology and Food Technology 0104 chemical sciences Biochemistry biology.protein Recombinant DNA bacteria 0210 nano-technology General Agricultural and Biological Sciences |
| Zdroj: | Tropical Life Sciences Research. 27:145-150 |
| ISSN: | 2180-4249 1985-3718 |
| DOI: | 10.21315/tlsr2016.27.3.20 |
| Popis: | We report on the cloning of the lipase gene from Bacillus licheniformis IBRL-CHS2 and the expression of the recombinant lipase. DNA sequencing analysis of the cloned lipase gene showed that it shares 99% identity with the lipase gene from B. licheniformis ATCC 14580 and belongs to subfamily 1.4 of true lipases based on amino acid sequence alignment of various Bacillus lipases. The 612 bp lipase gene was then cloned into the pET-15b(+) expression vector and the construct was transformed into E. coli BL21 (DE3) for bulk expression of the lipase. Expression was analysed by SDS-PAGE where the lipase was found to have a molecular weight of about 23 kDa. |
| Databáze: | OpenAIRE |
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