Transport of Schisandra chinensis extract and its biologically-active constituents across Caco-2 cell monolayers — an in-vitro model of intestinal transport
| Autor: | Shabana I. Khan, Young W Choi, Bharathi Avula, Vamsi L M Madgula, Larry A. Walker, Srinivas V. Pullela, Ikhlas A. Khan |
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| Rok vydání: | 2008 |
| Předmět: |
Schisandra chinensis
Biological Availability Pharmaceutical Science Dioxoles Models Biological High-performance liquid chromatography Lignans Permeability Diffusion Cyclooctanes chemistry.chemical_compound Humans Drug Interactions Polycyclic Compounds ATP Binding Cassette Transporter Subfamily B Member 1 Intestinal Mucosa Medicine Chinese Traditional Chromatography High Pressure Liquid Schisandra Pharmacology Chromatography biology Plant Extracts Chemistry Biological Transport Transporter Biological activity biology.organism_classification Intestines Biochemistry Caco-2 Gomisin A Efflux Caco-2 Cells Multidrug Resistance-Associated Proteins |
| Zdroj: | Journal of Pharmacy and Pharmacology. 60:363-370 |
| ISSN: | 2042-7158 0022-3573 |
| DOI: | 10.1211/jpp.60.3.0012 |
| Popis: | We have determined the intestinal transport of Schisandra chinensis extract and its lignans (gomisin A, gomisin N and schisandrin C) in the Caco-2 cell monolayer model. The transport across monolayers was examined for 2 h in absorptive and secretory directions. Quantitation of lignans was performed by HPLC. Out of the three lignans, gomisin A exhibited bi-directional transport, with Papp values in the range of 25–29 × 10−6 cm s−1, indicating a passive diffusion. Gomisin N, mixture and Schisandra extract displayed a higher transport in the secretory direction with efflux ratios in the range of 2.2–5.2. The efflux was decreased in the presence of inhibitors of multidrug resistance protein (MRP) transporter (MK-571) and P-glycoprotein (verapamil) indicating a possible involvement of an efflux pump and MRP in the transport of Schisandra lignans. Poor transport of schisandrin C was observed which could not be quantitated. The permeability of gomisin A in the isolated form was significantly different compared with the mixture or extract. |
| Databáze: | OpenAIRE |
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