Detection of Carbapenemase-Producing Enterobacteriaceae in Positive Blood Culture Using an Immunochromatographic RESIST-4 O.K.N.V. Assay

Autor:	Aurélie Cointe, André Birgy, Catherine Doit, Claire Amaris Hobson, Jeanne Truong, Audrey Monjault, Philippe Bidet, Stéphane Bonacorsi
Jazyk:	angličtina
Rok vydání:	2018
Předmět:	0301 basic medicine Carbapenemase-Producing Enterobacteriaceae 030106 microbiology Immunochromatographic test Coris Gene Expression Biology Sensitivity and Specificity Chromatography Affinity beta-Lactamases Microbiology Epidemiology and Surveillance 03 medical and health sciences Bacterial Proteins Humans Pharmacology (medical) Pharmacology Human blood Enterobacteriaceae Infections biology.organism_classification Enterobacteriaceae Isoenzymes Infectious Diseases Carbapenem-Resistant Enterobacteriaceae Blood Culture Positive blood culture Subculture (biology)
Popis:	We evaluated the performance of the RESIST-4 O.K.N.V. assay (Coris) with 98 isolates to detect OXA-48-like and KPC-, NDM-, and VIM-type carbapenemases directly on positive human blood cultures. OXA-48-like and KPC-type isolates were correctly detected, but the detection of NDM- and VIM-type carbapenemases was weak and variable. We show that repeating the test on a 4-h subculture improves the detection of NDM- and VIM-type carbapenemases to 100%.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2af0017878ed765ee75644a41b649d6e https://europepmc.org/articles/PMC6256770/ Zobrazit plný text záznamu