Time-resolved proteomics of adenovirus infected cells
| Autor: | Ulf Pettersson, Alberto Valdés, Sara Bergström Lind, Hongxing Zhao |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Phosphopeptides
Proteomics 0301 basic medicine Adenoviruses Integrins Proteome Adenoviridae Infections Gene Expression lcsh:Medicine Infektionsmedicin Pathology and Laboratory Medicine Biochemistry Tandem Mass Spectrometry Stable isotope labeling by amino acids in cell culture Medicine and Health Sciences Post-Translational Modification Phosphorylation Amino Acids lcsh:Science Cytoskeleton Multidisciplinary Messenger RNA Extracellular Matrix Cell biology Nucleic acids Medical Microbiology Viral Pathogens Viruses Pathogens Cellular Structures and Organelles Metabolic Networks and Pathways Research Article Infectious Medicine Biology Biosynthesis Microbiology Cell Line 03 medical and health sciences Virology DNA-binding proteins Genetics Cell Adhesion Humans Gene Regulation RNA Messenger Microbial Pathogens Transcription factor Gene Biology and life sciences Host Microbial Interactions Adenoviruses Human Host Cells lcsh:R Organisms Proteins RNA Cell Biology Regulatory Proteins Kinetics 030104 developmental biology Cell culture lcsh:Q DNA viruses Viral Transmission and Infection Transcription Factors |
| Zdroj: | PLoS ONE, Vol 13, Iss 9, p e0204522 (2018) PLoS ONE |
| Popis: | Viral infections cause large problems in the world and deeper understanding of the disease mechanisms is needed. Here we present an analytical strategy to investigate the host cell protein changes during human adenovirus type 2 (HAdV-C2 or Ad2) infection of lung fibro-blasts by stable isotope labelling of amino acids in cell culture (SILAC) and nanoLC-MS/MS. This work focuses on early phase of infection (6 and 12 h post-infection (hpi)) but the data is combined with previously published late phase (24 and 36 hpi) proteomics data to produce a time series covering the complete infection. As many as 2169 proteins were quantitatively monitored from 6 to 36 hpi, while some proteins were time-specific. After applying different filter criteria, 2027 and 2150 proteins were quantified at 6 and 12 hpi and among them, 431 and 544 were significantly altered at the two time points. Pathway analysis showed that the De novo purine and pyrimidine biosynthesis, Glycolysis and Cytoskeletal regulation by Rho GTPase pathways were activated early during infection while inactivation of the Integrin signalling pathway started between 6 and 12 hpi. Moreover, upstream regulator analysis predicted MYC to be activated with time of infection and protein and RNA data for genes controlled by this transcription factor showed good correlation, which validated the use of protein data for this prediction. Among the identified phosphorylation sites, a group related to glycolysis and cytoskeletal reorganization were up-regulated during infection. The results show specific aspects on how the host cell proteins, the final products in the genetic information flow, are influenced by Ad2 infection, which would be overlooked if only knowledge derived from mRNA data is considered. |
| Databáze: | OpenAIRE |
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