Phosphopeptide and phosphoprotein metabolism in brain
| Autor: | Marc Ledig, J.Y. Le Deaut, Paul Mandel |
|---|---|
| Rok vydání: | 1975 |
| Předmět: |
Phosphopeptides
Biophysics Biological Transport Active Biochemistry Oxidative Phosphorylation Ouabain chemistry.chemical_compound Adenosine Triphosphate Organophosphorus Compounds Microsomes Labelling Serine medicine Animals Molecular Biology Cerebral Cortex Phosphopeptide Brain Metabolism Phosphoproteins Rats chemistry Phosphoprotein Phosphoserine Microsome Phosphorylation Phosphorus Radioisotopes medicine.drug |
| Zdroj: | Biochimica et Biophysica Acta (BBA) - General Subjects. 381:324-336 |
| ISSN: | 0304-4165 |
| DOI: | 10.1016/0304-4165(75)90238-x |
| Popis: | Phosphopeptide and phosphoprotein phosphorylation was studied in rat brain microsomes and rat brain slices which were incubated in the presence of [γ-32P] ATP under various experimental conditions. Radioactive phosphoserine was isolated from phosphopeptides and phosphoproteins. Na+, K+, Mg2+ and cyclic AMP had a stimulating effect on the labelling of phosphopeptides. Ouabain and Ca2+ lowered the level of 32P incorporation into the phosphopeptides. The phosphoproteins behaved similarly to the phosphopeptides except for the potassium effect. Chase experiments showed a faster decrease in the labelling of phosphopeptides than in phosphoproteins. We suggest that both compounds may be involved in active transport phenomena. |
| Databáze: | OpenAIRE |
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