Tissue microarrays of bone marrow aspirate clot allow assessment of multiple samples
| Autor: | Maria Ap. Custodio Domingues, Pollyanna Domeny-Duarte, Ligia Niero |
|---|---|
| Přispěvatelé: | Universidade Estadual Paulista (Unesp) |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Pathology medicine.medical_specialty Tissue Fixation Biopsy Monoclonal Gammopathy of Undetermined Significance Pathology and Forensic Medicine Bone marrow clots 03 medical and health sciences 0302 clinical medicine Bone marrow aspirate Bone Marrow medicine Humans TMA Paraffin Embedding Tissue microarray Staining and Labeling business.industry Scientific production Medical school Cell Biology Immunohistochemistry Monoclonal gammopathy 030104 developmental biology medicine.anatomical_structure Tissue Array Analysis 030220 oncology & carcinogenesis Bone marrow Membrane staining medicine.symptom business |
| Zdroj: | Web of Science Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
| ISSN: | 0344-0338 |
| DOI: | 10.1016/j.prp.2019.152721 |
| Popis: | Made available in DSpace on 2020-12-10T19:55:03Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-01-01 Time optimization and cost reduction without quality loss is fundamental for scientific production. The TMA technique allows large-scale analysis of paraffin-embedded and fixed tissues using non-decalcified bone marrow specimens. Cytoinclusion samples (n = 264) were selected from patients diagnosed with monoclonal gammopathy (n = 189) at Botucatu Medical School, Sao Paulo State University. Slides were cut from the TMA block and stained with H&E and immunohistochemical markers. Of the 276 fragments included in the receptor block, 45 core detachments were observed, thus 84% of the samples remained viable. The representative material allowed for adequate analysis of isolated cells, which showed nuclear, cytoplasmic and membrane staining. Thus, we verified that inclusion of bone marrow aspirate clot is a valid alternative for TMA histological and immunohistochemical analyses. Sao Paulo State Univ, Botucatu Med Sch, Dept Pathol, BR-18618687 Botucatu, SP, Brazil Sao Paulo State Univ, Botucatu Med Sch, Dept Clin Med, BR-18618687 Botucatu, SP, Brazil Sao Paulo State Univ, Botucatu Med Sch, Dept Pathol, BR-18618687 Botucatu, SP, Brazil Sao Paulo State Univ, Botucatu Med Sch, Dept Clin Med, BR-18618687 Botucatu, SP, Brazil |
| Databáze: | OpenAIRE |
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