MicroRNA-7 downregulates the oncogene VDAC1 to influence hepatocellular carcinoma proliferation and metastasis
| Autor: | Lei Yin, Yong Qiang, Jiahui Chen, Yinda Wang, Lirong Zhu, Feiran Wang, Zhong Chen, Xian Shao, Yasu Jiang |
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| Rok vydání: | 2016 |
| Předmět: |
Adult
Male 0301 basic medicine Carcinoma Hepatocellular Blotting Western Down-Regulation Kaplan-Meier Estimate Biology Real-Time Polymerase Chain Reaction medicine.disease_cause Metastasis 03 medical and health sciences 0302 clinical medicine microRNA Biomarkers Tumor medicine Humans Neoplasm Invasiveness Aged Cell Proliferation Retrospective Studies Oncogene Voltage-Dependent Anion Channel 1 Liver Neoplasms General Medicine Middle Aged Prognosis medicine.disease Immunohistochemistry digestive system diseases Gene Expression Regulation Neoplastic MicroRNAs 030104 developmental biology Real-time polymerase chain reaction Tumor progression 030220 oncology & carcinogenesis Hepatocellular carcinoma Cancer research Female Carcinogenesis |
| Zdroj: | Tumor Biology. 37:10235-10246 |
| ISSN: | 1423-0380 1010-4283 |
| Popis: | Recent studies have been shown that voltage-dependent anion channel 1 (VDAC1) plays an important role in carcinogenesis. However, its molecular biological function in hepatocellular carcinoma (HCC) has not been entirely clarified. This study investigated the expression of VDAC1 in HCC and its prognostic value for HCC patients. Furthermore, we also identify the relevant VDAC1 direct target. Western blot, real-time quantitative PCR (qRT-PCR), and immunohistochemical (IHC) staining were performed to detect the expression of VDAC1 in HCC. Furthermore, the relationship between the VDAC1 level and clinicopathological features and prognostic values was explored. The effects of VDAC1 on HCC cell proliferation, migration, and invasion were also investigated in vitro. Predicted target gene of VDAC1 was determined by dual-luciferase reporter assay, qRT-PCR, and Western blot analyses. Our results revealed elevated VDAC1 messenger RNA (mRNA) (P = 0.0020) and protein (P = 0.0035) expression in tumor tissue samples compared with paired adjacent non-tumorous tissue samples. High VDAC1 expression was correlated with distant metastasis (P = 0.025), differentiation (P = 0.002), and advanced tumor stage (P = 0.004) in HCC patients. Kaplan-Meier survival analysis demonstrated that high expression of VDAC1 was significantly correlated with a poor prognosis for HCC patients (P |
| Databáze: | OpenAIRE |
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