Quantitative determination of Andrographolide and related compounds in Andrographis paniculata extracts and biological evaluation of their anti-inflammatory activity
| Autor: | Joana F. Campos, Sabine Berteina-Raboin, Véronique Ferreira, Emilie Destandau, Chantal Pichon, Emmanuelle Villedieu-Percheron |
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| Přispěvatelé: | Institut de Chimie Organique et Analytique (ICOA), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université d'Orléans (UO)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Centre de biophysique moléculaire (CBM), Université d'Orléans (UO)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Frapart, Isabelle, Proetos, Charlampos, Université d'Orléans (UO)-Centre National de la Recherche Scientifique (CNRS)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut de Chimie du CNRS (INC)-Institut National de la Santé et de la Recherche Médicale (INSERM), Université d'Orléans (UO)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC) |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Health (social science)
food.ingredient Andrographolide Plant Science Health Professions (miscellaneous) Microbiology 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine food reversed-phase liquid chromatography Chromatography detector [CHIM] Chemical Sciences [CHIM]Chemical Sciences anti-inflammatory activity Chromatography biology quantitative analysis Chemistry Extraction (chemistry) andrographolide method validation biology.organism_classification Terpenoid 3. Good health Andrographis Solvent 030220 oncology & carcinogenesis Quantitative analysis (chemistry) 030217 neurology & neurosurgery Andrographis paniculata Food Science |
| Zdroj: | Foods Foods, 2019, 8 (12), pp.683. ⟨10.3390/foods8120683⟩ The Benefits of Plant Extracts for Human Health Proetos, Charlampos. The Benefits of Plant Extracts for Human Health, MDPI Books, pp.129-140, 2021, 978-3-03943-851-8. ⟨10.3390/books978-3-03943-852-5⟩ Foods, MDPI, 2019, 8 (12), pp.683. ⟨10.3390/foods8120683⟩ Volume 8 Issue 12 |
| ISSN: | 2304-8158 |
| DOI: | 10.3390/foods8120683⟩ |
| Popis: | Extraction, isolation and characterization of Andrographis paniculata (A.p.) products were developed. Three natural diterpenes compounds were obtained and one was used for chemical modifications. Evaluation of their inhibition of TNF&alpha induced NF&kappa B transcriptional activity. A rapid analytical method for the determination and quantitation of three diterpenoid lactones (andrographolide 1, didehydroandrographolide 2, neoandrographiside 3) found in A. paniculata extracts was investigated. After some optimizations on column type and injection solvent, the separation was achieved in 9 min on a monolithic Chromolith Performance RP18e column (100 mm × 4.6 mm ID, 2 µ m), with a gradient solvent system of water and methanol, UV detection at 220 nm and ELSD detection. The method was proved to be suitable for the quantitation of these three diterpenes in four different commercial Andrographis dietary supplements. The anti-inflammatory activities of a mixture of known composition have been evaluated showing differences in activity depending on the relative ratio of various diterpenes and also a possible synergic activity for some of them. |
| Databáze: | OpenAIRE |
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