Tropism Analysis of Two Coxsackie B5 Strains Reveals Virus Growth in Human Primary Pancreatic Islets but not in Exocrine Cell Clusters In Vitro
| Autor: | Agneta Lukinius, Gun Frisk, Monika Hodik, Olle Korsgren |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
viruses
pancreatitis 030209 endocrinology & metabolism Enteroendocrine cell Biology human enterovirus capsid protein 1 medicine.disease_cause Virus Article 03 medical and health sciences 0302 clinical medicine medicine Coxsackie B virus pancreas Tropism 030304 developmental biology 0303 health sciences Pancreatic islets type 1 diabetes Virology In vitro 3. Good health medicine.anatomical_structure human islets of langerhans Viral replication Pancreas human enterovirus |
| Zdroj: | The Open Virology Journal The Open Virology Journal; Vol 7 |
| ISSN: | 1874-3579 |
| Popis: | Human Enteroviruses (HEVs) have been implicated in human pancreatic diseases such as pancreatitis and type 1 diabetes (T1D). Human studies are sparse or inconclusive and our aim was to investigate the tropism of two strains of Coxsackie B virus 5 (CBV-5) in vitro to primary human pancreatic cells. Virus replication was measured with TCID50 titrations of aliquots of the culture medium at different time points post inoculation. The presence of virus particles or virus proteins within the pancreatic cells was studied with immunohistochemistry (IHC) and electron microscopy (EM). None of the strains replicated in the human exocrine cell clusters, in contrast, both strains replicated in the endocrine islets of Langerhans. Virus particles were found exclusively in the endocrine cells, often in close association with insulin granules. In conclusion, CBV-5 can replicate in human endocrine cells but not in human exocrine cells, thus they might not be the cause of pancreatitis in humans. The association of virus with insulin granules might reflect the use of these as replication scaffolds. |
| Databáze: | OpenAIRE |
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