ACGT-containing abscisic acid response element (ABRE) and coupling element 3 (CE3) are functionally equivalent
| Autor: | Mihoko Asada, Tokunori Hobo, Tsukaho Hattori, Yasuo Kowyama |
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| Rok vydání: | 1999 |
| Předmět: |
Molecular Sequence Data
Response element Plant Science Plasma protein binding Computational biology Biology Response Elements chemistry.chemical_compound Gene Expression Regulation Plant Gene expression otorhinolaryngologic diseases Genetics Gene Abscisic acid Plant Proteins Regulation of gene expression Base Sequence food and beverages Promoter Cell Biology DNA-Binding Proteins Basic-Leucine Zipper Transcription Factors chemistry Regulatory sequence Trans-Activators Abscisic Acid Protein Binding Transcription Factors |
| Zdroj: | The Plant Journal. 19:679-689 |
| ISSN: | 1365-313X 0960-7412 |
| DOI: | 10.1046/j.1365-313x.1999.00565.x |
| Popis: | ACGT-containing ABA response elements (ABREs) have been functionally identified in the promoters of various genes. In addition, single copies of ABRE have been found to require a cis-acting, coupling element to achieve ABA induction. A coupling element 3 (CE3) sequence, originally identified as such in the barley HVA1 promoter, is found approximately 30 bp downstream of motif A (ACGT-containing ABRE) in the promoter of the Osem gene. The relationship between these two elements was further defined by linker-scan analyses of a 55 bp fragment of the Osem promoter, which is sufficient for ABA-responsiveness and VP1 activation. The analyses revealed that both motif A and CE3 sequence were required not only for ABA-responsiveness but also for VP1 activation. Since the sequences of motif A and CE3 were found to be similar, motif-exchange experiments were carried out. The experiments demonstrated that motif A and CE3 were interchangeable by each other with respect to both ABA and VP1 regulation. In addition, both sequences were shown to be recognized by a VP1-interacting, ABA-responsive bZIP factor TRAB1. These results indicate that ACGT-containing ABREs and CE3 are functionally equivalent cis-acting elements. Furthermore, TRAB1 was shown to bind two other non-ACGT ABREs. Based on these results, all these ABREs including CE3 are proposed to be categorized into a single class of cis-acting elements. |
| Databáze: | OpenAIRE |
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