Development of Chromosome Segment Substitution Lines (CSSLs) Derived from Guangxi Wild Rice (Oryza rufipogon Griff.) under Rice (Oryza sativa L.) Background and the Identification of QTLs for Plant Architecture, Agronomic Traits and Cold Tolerance
| Autor: | Liang Luo, Yufen Qin, Babar Usman, Shanyue Liao, Neng Zhao, Gul Nawaz, Ruizhi Yuan, Rongbai Li |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine lcsh:QH426-470 Quantitative trait locus Biology 01 natural sciences Genome quantitative trait locus (QTL) 03 medical and health sciences Genetics cold tolerance (CT) Cultivar Allele Indel Genetics (clinical) Oryza sativa rice food and beverages biology.organism_classification Oryza rufipogon lcsh:Genetics 030104 developmental biology marker-assisted selection (MAS) Backcrossing chromosome segment substitution lines (CSSLs) 010606 plant biology & botany |
| Zdroj: | Genes Volume 11 Issue 9 Genes, Vol 11, Iss 980, p 980 (2020) |
| ISSN: | 2073-4425 |
| DOI: | 10.3390/genes11090980 |
| Popis: | Common wild rice contains valuable resources of novel alleles for rice improvement. It is well known that genetic populations provide the basis for a wide range of genetic and genomic studies. In particular, chromosome segment substitution lines (CSSLs) ais a powerful tool for fine mapping of quantitative traits, new gene discovery and marker-assisted breeding. In this study, 132 CSSLs were developed from a cultivated rice (Oryza sativa) cultivar (93-11) and common wild rice (Oryza rufipogon Griff. DP30) by selfing-crossing, backcrossing and marker-assisted selection (MAS). Based on the high-throughput sequencing of the 93-11 and DP30, 285 pairs of Insertion-deletions (InDel) markers were selected with an average distance of 1.23 Mb. The length of this DP30-CSSLs library was 536.4 cM. The coverage rate of substitution lines cumulatively overlapping the whole genome of DP30 was about 91.55%. DP30-CSSLs were used to analyze the variation for 17 traits leading to the detection of 36 quantitative trait loci (QTLs) with significant phenotypic effects. A cold-tolerant line (RZ) was selected to construct a secondary mapping F2 population, which revealed that qCT2.1 is in the 1.7 Mb region of chromosome 2. These CSSLs may, therefore, provide powerful tools for genome wide large-scale gene discovery in wild rice. This research will also facilitate fine mapping and cloning of QTLs and genome-wide study of wild rice. Moreover, these CSSLs will provide a foundation for rice variety improvement. |
| Databáze: | OpenAIRE |
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