Lipid analysis by ion mobility spectrometry combined with mass spectrometry: A brief update with a perspective on applications in the clinical laboratory

Autor: Joshua Dubland
Jazyk: angličtina
Rok vydání: 2021
Předmět:
PC
glycerophosphocholine
TIMS
trapped ion mobility spectrometry
MALDI
matrix-assisted laser desorption/ionization
Clinical Biochemistry
FIA
flow injection analysis
TWIMS
traveling wave ion mobility spectrometry
CVD
cardiovascular disease
Clinical analysis
PE
phosphatidylethanolamine
PG
phosphatidylglycerol
TOF
time-of-flight
Microbiology
Special issue on Lipidomics
NBS
newborn screening
DMS
differential mobility spectrometry
SLIM
structures for loss less ion manipulations
HRMS
high-resolution mass spectrometry
Medical technology
DG
diacylglycerol
FTICR
fourier-transform ion cyclotron resonance
SV
separation voltage
R855-855.5
CV
compensation voltage
RF
radio frequency
Spectroscopy
DTIMS
drift tube ion mobility spectrometry
LPC
lysophosphatidylcholine
SM
sphingomyelin
TG
triglyceride
Mass spectrometry
LC
liquid chromatography
IMS
ion mobility spectrometry
VLDL
very-low-density lipoprotein
CCS
collisional cross section
FAIMS
field asymmetric waveform ion mobility spectrometry
m/z
mass-to-charge ratio
Ion mobility spectrometry
HDL
high-density-lipoprotein
LDL
low-density-lipoprotein
Lipids
EV
elution voltage
Medical Laboratory Technology
MS
mass spectrometry
IMS-MS
ion mobility spectrometry-mass spectrometry
Zdroj: Journal of Mass Spectrometry and Advances in the Clinical Lab
Journal of Mass Spectrometry and Advances in the Clinical Lab, Vol 23, Iss, Pp 7-13 (2022)
ISSN: 2667-145X
2667-1468
Popis: Ion mobility spectrometry (IMS) is an analytical technique where ions are separated in the gas phase based on their mobility through a buffer gas in the presence of an electric field. An ion passing through an IMS device has a characteristic collisional cross section (CCS) value that depends on the buffer gas used. IMS can be coupled with mass spectrometry (MS), which characterizes an ion based on a mass-to-charge ratio (m/z), to increase analytical specificity and provide further physicochemical information. In particular, IMS-MS is of ever-increasing interest for the analysis of lipids, which can be problematic to accurately identify and quantify in bodily fluids by liquid chromatography (LC) with MS alone due to the presence of isomers, isobars, and structurally similar analogs. IMS provides an additional layer of separation when combined with front-end LC approaches, thereby, enhancing peak capacity and analytical specificity. CCS (and also ion mobility drift time) can be plotted against m/z ion intensity and/or LC retention time in order to generate in-depth molecular profiles of a sample. Utilization of IMS-MS for routine clinical laboratory testing remains relatively unexplored, but areas do exist for potential implementation. A brief update is provided here on lipid analysis using IMS-MS with a perspective on some applications in the clinical laboratory.
Databáze: OpenAIRE
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