Human Marrow Stromal Cell Treatment Provides Long-lasting Benefit after Traumatic Brain Injury in Rats
| Autor: | Michael Chopp, Changsheng Qu, Dunyue Lu, Anton Goussev, Asim Mahmood |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Male
Long lasting Pathology medicine.medical_specialty Indoles Time Factors Stromal cell Traumatic brain injury Human bone Bone Marrow Cells Motor Activity Article Stroma Glial Fibrillary Acidic Protein medicine Animals Humans Rats Wistar Bone Marrow Transplantation Neurologic Examination Glial fibrillary acidic protein biology business.industry Phosphopyruvate hydratase Nuclear Proteins Recovery of Function equipment and supplies medicine.disease Immunohistochemistry Rats Brain Injuries Phosphopyruvate Hydratase biology.protein Surgery Neurology (clinical) Stromal Cells business |
| Zdroj: | Neurosurgery. 57:1026-1031 |
| ISSN: | 1524-4040 0148-396X |
| DOI: | 10.1227/01.neu.0000181369.76323.50 |
| Popis: | This study was designed to investigate the effects of human bone marrow stromal cell (hMSC) administration in rats for 3 months after traumatic brain injury (TBI).Adult male Wistar rats (n = 60) were injured with controlled cortical impact and divided into four groups. The three treatment groups (n = 10 each) were injected with 2 x 10, 4 x 10, and 8 x 10 hMSCs, respectively, intravenously, whereas the control group (n = 30) received phosphate-buffered saline. All injections were performed 1 day after injury into the tail veins of rats. Neurological functional evaluation of animals was performed before and after injury by use of Neurological Severity Scores. Animals were sacrificed 3 months after TBI, and brain sections were stained by immunohistochemistry.Statistically significant improvement in functional outcome was observed in all three treatment groups compared with control values (P0.05). This benefit was visible 14 days after TBI and persisted until 3 months (end of trial). There was no difference in functional outcome among the three treatment groups. Histological analysis showed that hMSCs were present in the lesion boundary zone at 3 months with all three doses tested.hMSCs injected in rats after TBI survive until 3 months and provide long-lasting functional benefit. Functional improvement may be attributed to stimulation of endogenous neurorestorative functions such as neurogenesis and synaptogenesis. |
| Databáze: | OpenAIRE |
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