The investigation of adrenal involvement in carbapenem resistant Acinetobacter baumannii sepsis: experimental mouse model
| Autor: | Olgun Kontaş, Mehmet Doganay, Emine Alp Meşe, Esma Eren, Gökçen Dinç |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Acinetobacter baumannii
medicine.medical_treatment Physiology Inflammation Bacteremia Microbial Sensitivity Tests Sepsis chemistry.chemical_compound Mice Corticosterone medicine Adrenal insufficiency Animals biology Adrenal gland business.industry General Medicine medicine.disease biology.organism_classification Anti-Bacterial Agents medicine.anatomical_structure Cytokine Carbapenem-Resistant Enterobacteriaceae chemistry Carbapenems Tumor necrosis factor alpha Female medicine.symptom business Acinetobacter Infections Adrenal Insufficiency |
| Popis: | The investigation of adrenal involvement in carbapenem resistant Acinetobacter baumannii sepsis: experimental mouse model Abstract Background/Aim: In the last years, incidence of carbapenem resistant Acinetobacter baumannii sepsis is increasing with high mortality. However, it is not clear whether this is due to inadequate antimicrobial choice or a more severe clinical course. We aimed to evaluate the inflammation and adrenal involvement in the carbapenem resistant A. baumannii by using experimental mouse model sepsis. METHOD Balb/c female mice were randomly put into control and three sepsis groups (A. baumannii susceptible to carbapenem-CSAB-, A. baumannii resistant to carbapenem-CRAB-, Escherichia coli). A total of sixty mice were included in this study with each group having 15 mice. Mice were sacrificed, 72 hours after bacterial inoculation and blood was taken from each mouse for the assessment of cytokines and corticosterone. Both adrenal glands were dissected; one was used for culture and the other was used for histopathological examination. Bacterial loads of organs were calculated as CFU/g. The histopathological changes, bacterial levels in adrenal and cytokine and corticosterone levels were assessed and compared among the groups. RESULTS The bacterial level was higher in E. coli (108,45 ±30,55 log10 CFU/g) (mean±SD) than other sepsis groups. The lowest level of corticosterone was observed in the E. coli group (p |
| Databáze: | OpenAIRE |
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