Identification and characterization of a novel antimicrobial peptide compound produced by Bacillus megaterium strain isolated from oral microflora
| Autor: | Adel Fathi, Saleh B. Al Ghamdi, Mohammed A.S. Abourehab, Abdullah Safar Althubiani, Mohammed Alarjah, Yahia Ahmed Maher, Mohd Sajjad Ahmad Khan |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Gram-negative bacteria 030106 microbiology Pharmaceutical Science Bacitracin medicine.disease_cause Article Microbiology 03 medical and health sciences Minimum inhibitory concentration chemistry.chemical_compound medicine Escherichia coli Bacillus megaterium Pharmacology biology Chemistry lcsh:RM1-950 biology.organism_classification Antimicrobial Bioactive compound 030104 developmental biology lcsh:Therapeutics. Pharmacology bacteria Micrococcus luteus medicine.drug |
| Zdroj: | Saudi Pharmaceutical Journal, Vol 26, Iss 8, Pp 1089-1097 (2018) |
| ISSN: | 1319-0164 |
| Popis: | In recent years, the decreased efficacy of existing antibiotics toward management of emergent drug-resistant strains has necessitated the search for novel antibiotics from natural products. In this regard, Bacillus sp is well known for producing variety of secondary metabolites of potential use. Therefore, we performed an investigation to isolate and identify Bacillus sp from oral cavity for production of novel antimicrobial compounds. We extracted, purified, and identified a novel bioactive compound by B. megaterium (KC246043.1). The optimal production of compound was observed on de Man Rogosa and Sharpe broth by incubating at 37 °C, and pH 7.0 for 4 days. The bioactive compound was extracted by using n-butanol (2:1 v/v), purified on TLC plates with detection at Rf 7.8 cm; further characterized and identified as a cyclic ploypeptide sharing structural similarity with bacitracin. Minimum inhibitory concentration of bioactive compound was found to be 0.25, 0.5, 1.0, 3.125 and 6.25 μg/ml against Micrococcus luteus ATCC10240, Salmonella typhi ATCC19430, Escherichia coli ATCC35218. Pseudomonas aeruginosa ATCC27853 and Staphylococcus aureus ATCC25923 respectively, with no activity against Candida albicans ATCC10231. Our findings have revealed a novel cyclic peptide compound from B. megaterium with broad spectrum antimicrobial activity against both Gram positive and Gram negative bacteria. Keywords: Antibacterial, Bacillus megaterium, Bacitracin, Drug discovery, Peptide antibiotics |
| Databáze: | OpenAIRE |
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