Generation of inner ear sensory neurons using blastocyst complementation in a Neurog1 +/−−deficient mouse
| Autor: | Peter A. Santi, Walter C. Low, Aleta R. Steevens, Yun You, James R. Dutton, Matthew W. Griesbach |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Cell type
Medicine (miscellaneous) Stem cells Biology Biochemistry Genetics and Molecular Biology (miscellaneous) Loss of heterozygosity lcsh:Biochemistry Blastocyst complementation Inner ear medicine otorhinolaryngologic diseases lcsh:QD415-436 Blastocyst Induced pluripotent stem cell lcsh:R5-920 Heterozygote advantage Cell Biology Neurogenin1 Cell biology Complementation medicine.anatomical_structure embryonic structures Regenerative medicine Molecular Medicine sense organs Stem cell Spiral ganglion neurons lcsh:Medicine (General) |
| Zdroj: | Stem Cell Research & Therapy, Vol 12, Iss 1, Pp 1-12 (2021) |
| ISSN: | 1757-6512 |
| Popis: | This research is the first to produce induced pluripotent stem cell-derived inner ear sensory neurons in the Neurog1+/− heterozygote mouse using blastocyst complementation. Additionally, this approach corrected non-sensory deficits associated with Neurog1 heterozygosity, indicating that complementation is specific to endogenous Neurog1 function. This work validates the use of blastocyst complementation as a tool to create novel insight into the function of developmental genes and highlights blastocyst complementation as a potential platform for generating chimeric inner ear cell types that can be transplanted into damaged inner ears to improve hearing. |
| Databáze: | OpenAIRE |
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