The influence of different VEGF administration protocols on the perfusion of epigastric flaps in rats
| Autor: | Anna Borgmann, Stefan Wagenpfeil, Marco R. Kesting, Klaus-Dietrich Wolff, Thomas Mücke, David A. Mitchell, Andreas M. Fichter, Lucas M. Ritschl |
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| Rok vydání: | 2012 |
| Předmět: |
Vascular Endothelial Growth Factor A
medicine.medical_specialty Necrosis Time Factors Injections Subcutaneous Neovascularization Physiologic Free flap Free Tissue Flaps Surgical Flaps chemistry.chemical_compound Hemoglobins Random Allocation Oxygen Consumption medicine Laser-Doppler Flowmetry Animals Oxygen saturation (medicine) business.industry Graft Survival Blood flow Epigastric Arteries Confidence interval Rats Inbred F344 Surgery Capillaries Rats Vascular endothelial growth factor Otorhinolaryngology chemistry Regional Blood Flow Spectrophotometry Microvessels Granulation Tissue Oral Surgery medicine.symptom Ligation business Perfusion Blood Flow Velocity |
| Zdroj: | The British journal of oralmaxillofacial surgery. 51(6) |
| ISSN: | 1532-1940 |
| Popis: | In this study we investigated the impact of the time at which vascular endothelial growth factor (VEGF) was given on the overall survival of flaps and time of the achievement of full perfusion in the rat epigastric flap model. Epigastric flaps were raised in 144 rats. VEGF was given to 108 rats at different time points. Perioperatively blood flow, velocity, haemoglobin (Hb) concentration, and oxygen saturation were measured non-invasively using laser-Doppler spectrophotometry. On postoperative days 3, 5, 7, or 10, the pedicle of the flap was ligated. One week later, the flap was harvested for immunohistochemical assessment of the density of microvessels. Differences significant from those in the control group were found in all specimens 3 and 5 days after the first operation, whereas no differences in the area of necrosis were detected after 7 and 10 days. Preoperative values of measured Hb (p |
| Databáze: | OpenAIRE |
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