Plasma and follicular fluid osteopontin levels during ovarian cycle and their correlation with follicular fluid vascular endothelial growth factor levels
| Autor: | Toshiyuki Takeshita, Yoshimitsu Kuwabara, Akira Katayama, Shuichi Ono, Sachiko Kurihara, Yumiko Oishi |
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| Rok vydání: | 2021 |
| Předmět: |
Adult
Vascular Endothelial Growth Factor A 0301 basic medicine 010504 meteorology & atmospheric sciences Physiology Science medicine.medical_treatment Ovarian hyperstimulation syndrome Stimulation 01 natural sciences Article Andrology Ovarian Hyperstimulation Syndrome 03 medical and health sciences chemistry.chemical_compound Endocrinology Medical research stomatognathic system medicine Humans Osteopontin Menstrual Cycle 0105 earth and related environmental sciences Multidisciplinary In vitro fertilisation biology Oocyte medicine.disease Follicular fluid Pathophysiology Follicular Fluid Vascular endothelial growth factor 030104 developmental biology medicine.anatomical_structure chemistry biology.protein Medicine Female Biomarkers |
| Zdroj: | Scientific Reports, Vol 11, Iss 1, Pp 1-6 (2021) Scientific Reports |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-020-79453-1 |
| Popis: | Osteopontin (OPN) is a multifunctional secreted glycoprotein. We evaluated OPN concentrations in blood and follicular fluid (FF) during the ovarian cycle and their relationship with the production of vascular endothelial growth factor (VEGF), which is involved in the pathophysiology of ovarian hyperstimulation syndrome (OHSS). Twenty-two women undergoing in vitro fertilization (minimal stimulation protocol with clomiphene citrate) were enrolled. Samples were collected (a) on the third day of withdrawal bleeding, (b) 2 days before oocyte retrieval, and (c) on the day of oocyte retrieval. FF was collected during oocyte retrieval. The OPN concentration in each specimen and the VEGF concentration in FF was measured by enzyme-linked immunosorbent assays. Plasma OPN concentrations were (in ng/mL): (a) 416 ± 37.2, (b) 378 ± 35.8, and (c) 390 ± 40.0, with no significant differences between the groups. The OPN concentration in FF was 106 ± 13.4 ng/mL. A positive correlation was found between OPN concentrations in FF and plasma samples. A positive correlation was also found between plasma OPN and FF VEGF concentrations, irrespective of the blood-sampling period. Plasma OPN concentration is suggested to reflect the FF VEGF level at oocyte retrieval and maybe a novel clinical marker for predicting the risk for OHSS. |
| Databáze: | OpenAIRE |
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