Effect of Tannic Acid on the Protective Properties of the in situ Formed Pellicle
| Autor: | Matthias Hannig, Christian Hannig, Sabine Basche, Susann Hertel, Sandra Pötschke, Wiebke Hoth-Hannig, Judith Delius |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
In situ Adult Calcium Phosphates Dental pellicle Mouthwashes macromolecular substances Dental Caries Bacterial Adhesion Statistics Nonparametric Enamel Pellicle Streptococcus mutans 03 medical and health sciences chemistry.chemical_compound Fluorides 0302 clinical medicine Bioadhäsion Erosion Pellicle Polyphenole Gerbsäure Microscopy Electron Transmission Tannic acid Animals Humans Tooth Erosion Dental Pellicle ddc:610 Bioadhesion Erosion Pellicle Polyphenols Tannic acid General Dentistry Glucans biology Dose-Response Relationship Drug Chemistry Biofilm 030206 dentistry Hydrogen-Ion Concentration biology.organism_classification 030104 developmental biology Biochemistry Microscopy Fluorescence Polyphenol Biofilms Cattle Tannins Ex vivo Nuclear chemistry |
| Popis: | Objectives: In the present in situ/ex vivo study the impact of tannic acid on the erosion-protective properties of the enamel pellicle was tested. Additionally, the antiadherent and antibacterial effects of tannic acid were evaluated. Methods: The pellicle was formed in situ on bovine enamel samples fixed on individual splints worn by 6 subjects. Following 1 min of pellicle formation the volunteers rinsed for 10 min with tannic acid. After further oral exposure for 19 min, 109 min, and 8 h overnight, respectively, slabs were incubated in HCl ex vivo (pH 2.0, 2.3, 3.0) over 120 s. Subsequently, kinetics of calcium and phosphate release were measured photometrically. Samples after a 1-min fluoride mouth rinse as well as enamel samples with and without a 30-min in situ pellicle served as controls. Antiadherent effects were evaluated after a 1-min rinse with tannic acid and oral exposure of the slabs overnight. DAPI (4′,6-diamidino-2-phenylindole) combined with concanavalin A staining and live/dead staining was used for fluorescence microscopic visualization and quantification of adherent bacteria and glucans. Modification of the pellicle's ultrastructure by tannic acid was evaluated by transmission electron microscopy (TEM). Results: Tannic acid significantly improved the erosion-protective properties of the pellicle in a pH-dependent manner. Bacterial adherence and glucan formation on enamel were significantly reduced after rinses with tannic acid as investigated by fluorescence microscopy. TEM imaging indicated that rinsing with tannic acid yielded a sustainable modification of the pellicle; it was distinctly more electron dense. Conclusion: Tannic acid offers an effective and sustainable approach for the prevention of caries and erosion. |
| Databáze: | OpenAIRE |
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