Identification of a cell wall-associated subtilisin-like serine protease involved in the pathogenesis of Streptococcus suis serotype 2
| Autor: | Zhengjun Yu, Liu Teng, Mingguang Zhou, Jun Li, Peng Liu, Qiaoyun Hu, Meilin Jin, Weicheng Bei, Huanchun Chen, Gang Zhao |
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| Rok vydání: | 2010 |
| Předmět: |
Streptococcus suis
Swine medicine.medical_treatment Mutant Virulence Microbiology Virulence factor Bacterial Proteins Cell Wall Streptococcal Infections medicine Animals Humans Swine Diseases Serine protease Streptococcus suis serotype 2 Protease biology Reverse Transcriptase Polymerase Chain Reaction Gene Expression Profiling fungi Subtilisin Wild type Membrane Proteins biology.organism_classification Survival Analysis Molecular biology Mutagenesis Insertional Infectious Diseases Genes Bacterial biology.protein Gene Deletion |
| Zdroj: | Microbial Pathogenesis. 48:103-109 |
| ISSN: | 0882-4010 |
| DOI: | 10.1016/j.micpath.2009.11.005 |
| Popis: | Streptococcus suis is an important swine and human pathogen, and also an emerging zoonotic agent. A surface-associated subtilisin-like serine protease (SspA) of S. suis was identified by screening a genomic expression library as fragments of this protein reacted most strongly with convalescent-phase pig sera. The sspA gene is present in 29 of 33 S. suis serotypes reference strains and is expressed on the surface of S. suis. Relative real-time quantitative PCR assay demonstrated that sspA mRNA expression in vivo was several thousand fold of that in vitro. A sspA(-) mutant was generated from a S. suis serotype 2 strain SC19 by allelic exchange. The mutant was not different from the wild type strain in subcellular structures and in hemolytic phenotype. However, the virulence of the sspA(-) mutant was markedly lower than the wild type in pigs as demonstrated in experimental infections. These data indicated that the surface-associated protein SspA is a conserved virulence factor of S. suis and is involved in the pathogenesis of S. suis. |
| Databáze: | OpenAIRE |
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