Pepsin Digest of Gliadin Forms Spontaneously Amyloid-Like Nanostructures Influencing the Expression of Selected Pro-Inflammatory, Chemoattractant, and Apoptotic Genes in Caco-2 Cells: Implications for Gluten-Related Disorders
| Autor: | Nicolo Tonali, Karen M Lammers, Andreas Hütten, Maria Georgina Herrera, Verónica I. Dodero, Philipp W Dörfler, Marion Gras, Inga Ennen, Francesco Nicoletti, Yvonne Hannappel, Thomas Hellweg |
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| Rok vydání: | 2021 |
| Předmět: |
Inflammation
Apoptosis digestive system Gliadin Protein Structure Secondary Immune system Pepsin medicine Humans Protein Structure Quaternary biology Chemotactic Factors Chemistry food and beverages nutritional and metabolic diseases Chemotaxis digestive system diseases Pepsin A Nanostructures Celiac Disease Biochemistry Gene Expression Regulation Caco-2 Cell culture Proteolysis biology.protein medicine.symptom Caco-2 Cells Food Science Biotechnology |
| Zdroj: | Molecular nutritionfood research. 65(16) |
| ISSN: | 1613-4133 |
| Popis: | SCOPE: Proteolysis-resistant gliadin peptides are intensely investigated in biomedical research related to Celiac Disease and gluten-related disorders. Herein, the first integrated supramolecular investigation of pepsin-digested gliadin peptides, p-gliadin, is presented in combination with its functional behavior in Caco-2 cell line.; METHODS AND RESULTS: First, we investigated gliadin degradation by pepsin at pH 3, and the physicochemical properties of p-gliadin were compared with gliadin. An integrated approach using interfacial, spectroscopic, and microscopic techniques revealed that the p-gliadin forms spontaneously soluble large supramolecular structures, mainly oligomers and fibrils capable of binding amyloid-sensitive dyes. The self-assembly of p-gliadin starts at a concentration of 0.40mug/ml. Second, we stimulated CaCo-2 cells with the p-gliadin supramolecular system and screened the mRNA expression levels of a panel of genes involved in cellular inflammation, apoptosis, permeability, and chemoattraction of immune cells. Our findings suggest that p-gliadin composed of supramolecular structures triggers significant mRNA up-regulation (p |
| Databáze: | OpenAIRE |
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