Application of a digital PCR method for WT1 to myeloid neoplasms in CR and deep ELN WT1 molecular response (10 copies)

Autor:	Miguel Ángel Rubio, Antoni Garcia, Jordi Esteve, Josep M. Sierra, Isabel Badell, Marta Pratcorona, Carmen Pedro, Maite Carricondo, Montserrat Arnan, L Sansegundo, Anna Monter, Josep F. Nomdedeu, Ferran Vall-Llovera, S Brunet, X Ortin, Mar Tormo, A Brell, Elena Bussaglia, J. Bargay, C. Talarn
Rok vydání:	2019
Předmět:	Oncology Adult Male medicine.medical_specialty Myeloid DNA Complementary Genes Wilms Tumor medicine.medical_treatment Gene Dosage Hematopoietic stem cell transplantation Kaplan-Meier Estimate Real-Time Polymerase Chain Reaction Polymerase Chain Reaction law.invention Immunophenotyping 03 medical and health sciences Young Adult 0302 clinical medicine law Internal medicine medicine Humans Digital polymerase chain reaction RNA Neoplasm Polymerase chain reaction Survival analysis Aged business.industry Infant Hematology General Medicine Middle Aged medicine.disease Flow Cytometry Prognosis Minimal residual disease Leukemia Leukemia Myeloid Acute medicine.anatomical_structure 030220 oncology & carcinogenesis Myelodysplastic Syndromes Female Bone marrow business 030215 immunology
Zdroj:	Annals of hematology. 99(4)
ISSN:	1432-0584
Popis:	Bone marrow WT1 mRNA levels assessed by the ELN method are useful to establish prognostic correlations in myeloid malignancies treated with chemotherapy or hematopoietic stem cell transplantation (HCT). Those patients with WT1 levels below ten copies have a good outcome. However, some of these patients relapse. To further characterize this group of cases, we applied a new and sensitive digital (ddPCR) WT1 method. A consecutive series of 49 patients with treated myeloid malignancies and with an ELN WT1 quantitation of
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2ad6dfdf36bbdf1865f27e3508c1a544 https://pubmed.ncbi.nlm.nih.gov/32062741 Zobrazit plný text záznamu Full text from SpringerLink