ParP prevents dissociation of CheA from chemotactic signaling arrays and tethers them to a polar anchor
| Autor: | Kathrin Schirner, Xiaoji Wu, Simon Ringgaard, Brigid M. Davis, Matthew K. Waldor, Martha A. Zepeda-Rivera |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
DNA Topoisomerase IV
Histidine Kinase Poly ADP ribose polymerase ATPase Methyl-Accepting Chemotaxis Proteins Flagellum medicine.disease_cause Time-Lapse Imaging Bacterial Proteins Signaling proteins Escherichia coli medicine Vibrio cholerae Multidisciplinary biology Chemotaxis Escherichia coli Proteins Cell Polarity Computational Biology Membrane Proteins biochemical phenomena metabolism and nutrition Protein subcellular localization prediction Cell biology Agar Microscopy Fluorescence PNAS Plus biology.protein bacteria Vibrio parahaemolyticus Fluorescence Recovery After Photobleaching Signal Transduction |
| Zdroj: | Proceedings of the National Academy of Sciences. 111 |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.1315722111 |
| Popis: | Bacterial chemotaxis proteins are organized into ordered arrays. In peritrichous organisms, such as Escherichia coli, stochastic assembly processes are thought to account for the placement of chemotaxis arrays, which are nonuniformly distributed. In contrast, we previously found that chemotactic signaling arrays in polarly flagellated vibrios are uniformly polar and that array localization is dependent on the ParA-like ATPase ParC. However, the processes that enable ParC to facilitate array localization have not been described. Here, we show that a previously uncharacterized protein, ParP, interacts with ParC and that ParP is integral to array localization in Vibrio parahaemolyticus. ParC's principal contribution to chemotaxis appears to be via positioning of ParP. Once recruited to the pole by ParC, ParP sequesters arrays at this site by capturing and preventing the dissociation of chemotactic signaling protein (CheA). Notably, ParP also stabilizes chemotactic protein complexes in the absence of ParC, indicating that some of its activity is independent of this interaction partner. ParP recruits CheA via CheA's localization and inheritance domain, a region found only in polarly flagellated organisms that encode ParP, ParC, and CheA. Thus, a tripartite (ParC-ParP-CheA) interaction network enables the polar localization and sequestration of chemotaxis arrays in polarly flagellated organisms. Localization and sequestration of chemotaxis clusters adjacent to the flagella--to which the chemotactic signal is transmitted--facilitates proper chemotaxis as well as accurate inheritance of these macromolecular machines. |
| Databáze: | OpenAIRE |
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