Cryopreservation and the Freeze–Thaw Stress Response in Yeast
| Autor: | Meaghan R. Robinson, Verónica A. Segarra, Elizabeth Cabrera, Candyce M Sturgeon, Mackenzie M. Crow, Laylah C Welch |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Saccharomyces cerevisiae Proteins lcsh:QH426-470 Cryoprotectant 030106 microbiology Saccharomyces cerevisiae Context (language use) Review yeast Cryopreservation Fight-or-flight response 03 medical and health sciences Freezing Genetics Viability assay Genetics (clinical) cryoprotectants biology biology.organism_classification Adaptation Physiological Yeast Cell biology lcsh:Genetics 030104 developmental biology freeze–thaw stress response Adaptation |
| Zdroj: | Genes Genes, Vol 11, Iss 835, p 835 (2020) |
| ISSN: | 2073-4425 |
| Popis: | The ability of yeast to survive freezing and thawing is most frequently considered in the context of cryopreservation, a practical step in both industrial and research applications of these organisms. However, it also relates to an evolved ability to withstand freeze–thaw stress that is integrated with a larger network of survival responses. These responses vary between different strains and species of yeast according to the environments to which they are adapted, and the basis of this adaptation appears to be both conditioned and genetic in origin. This review article briefly touches upon common yeast cryopreservation methods and describes in detail what is known about the biochemical and genetic determinants of cell viability following freeze–thaw stress. While we focus on the budding yeast Saccharomyces cerevisiae, in which the freeze–thaw stress response is best understood, we also highlight the emerging diversity of yeast freeze–thaw responses as a manifestation of biodiversity among these organisms. |
| Databáze: | OpenAIRE |
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