Brain antioxidant status in a high pressure–induced rat model of glaucoma
| Autor: | Susana Llesuy, Ricardo Brunzini, Claudia Reides, Sandra M. Ferreira, Fabian S Lerner |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Antioxidant
Luminescence medicine.medical_treatment Glaucoma Ciencias de la Salud Ascorbic Acid medicine.disease_cause Antioxidants Protein Carbonylation chemistry.chemical_compound ANTIOXIDANTS GLAUCOMA Vitamin E BRAIN OXIDATIVE STRESS chemistry.chemical_classification biology Glutathione peroxidase Brain General Medicine Catalase Glutathione Reactive Nitrogen Species Otras Ciencias de la Salud Biochemistry Female medicine.medical_specialty CIENCIAS MÉDICAS Y DE LA SALUD Superoxide dismutase Internal medicine medicine Animals Rats Wistar Intraocular Pressure Glutathione Peroxidase Superoxide Dismutase medicine.disease Ascorbic acid Rats Ophthalmology Disease Models Animal Oxidative Stress Endocrinology chemistry biology.protein Ocular Hypertension Trolox Reactive Oxygen Species Oxidative stress |
| DOI: | 10.1111/j.1755-3768.2012.02572.x/abstract |
| Popis: | PURPOSE: The goal of the present study is to establish the antioxidant status in the brain of a high pressure-induced rat model. METHODS: Ocular hypertension was induced in rats (n = 12) cauterizing two episcleral veins under a surgical microscope. A sham procedure (n = 12) was performed in the control group. The markers evaluated in the brain 7 days after surgery were as follows: spontaneous chemiluminescence, protein carbonylation, nitrite concentration, total reactive antioxidant potential (TRAP), ascorbic acid, glutathione, vitamin E and activities of superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase. RESULTS: Chemiluminescence in glaucoma was 55% higher than in controls (393 ± 20 cpm/mg protein, p < 0.001). Protein carbonylation in glaucoma was 93% higher than in controls (1.15 ± 0.18 nmol/mg protein, p < 0.001). Nitrite concentration was 5.30 ± 0.25 μM for glaucoma (controls 4.41 ± 0.24 μM, p < 0.05). Total reactive antioxidant potential decreased by 42% in glaucoma (controls 153 ± 14 μM Trolox, p < 0.001). Ascorbic acid was 67 ± 26 μM for glaucoma (controls 275 ± 22 μM, p < 0.001). Vitamin E was 0.58 ± 0.05 μmol/g organ for glaucoma (controls 1.10 ± 0.06 μmol/g organ, p < 0.01). Glutathione was 1.98 ± 0.13 μmol/g organ for glaucoma (controls 8.19 ± 0.71 μmol/g organ, p < 0.001). Superoxide dismutase and GPx were increased in glaucoma by 42 and 59%, respectively (p < 0.05). CONCLUSIONS: Reactive oxygen and nitrogen species were increased in glaucoma, the increase in chemiluminescence, protein carbonylation and nitrite levels could be evidenced by this situation. The decrease in nonenzymatic antioxidants and a compensatory increase in SOD and GPx activity may have been a consequence of an increase in oxidative processes. Fil: Ferreira, Sandra María. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica. Cátedra de Química General e Inorgánica; Argentina Fil: Lerner, Fabián. Fundación para la Investigación del Glaucoma; Argentina Fil: Reides, Claudia Gabriela. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica. Cátedra de Química General e Inorgánica; Argentina Fil: Brunzini, Ricardo. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica. Cátedra de Química General e Inorgánica; Argentina Fil: Llesuy, Susana Francisca. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica. Cátedra de Química General e Inorgánica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Programa de Radicales Libres; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina |
| Databáze: | OpenAIRE |
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