Standardization of Functional Reporter and Antibiotic Resistance Cassettes to Facilitate the Genetic Engineering of Filamentous Fungi
| Autor: | Olga Spassibojko, Swati Sureka, B. Gillian Turgeon, Arun Chakravorty, Dongliang Wu, Nupur Bhatt, Eric C. Holmes |
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| Rok vydání: | 2014 |
| Předmět: |
Genetics
biology Biomedical Engineering Drug Resistance Microbial General Medicine Cochliobolus heterostrophus BioBrick Transfection biology.organism_classification Biochemistry Genetics and Molecular Biology (miscellaneous) Aspergillus nidulans Microbiology chemistry.chemical_compound Antibiotic resistance Ascomycota chemistry Genes Reporter Microbial genetics Genetic Engineering Gene Hygromycin B Organism Plasmids |
| Zdroj: | ACS Synthetic Biology. 3:960-962 |
| ISSN: | 2161-5063 |
| DOI: | 10.1021/sb5000143 |
| Popis: | The unique physiological properties of fungi are useful for a myriad of applications, which could greatly benefit from increased control of native pathways and introduction of recombinant genes. However, fungal genetic engineering is still limited in scope and accessibility, largely due to lack of standardization. To help standardize the genetic engineering of filamentous fungi, we created BioBricks of commonly used antibiotic resistance genes, neomycin phosphotransferase (nptII) and hygromycin phosphotransferase (hph), which confer resistance to G418 (Geneticin) and hygromycin B, respectively. Additionally, we created a BioBrick of the constitutive trpC promoter, from the tryptophan biosynthesis pathway of Aspergillus nidulans, and used it to create a composite part including the GFP gene. The functionality of these parts was demonstrated in the model fungal organism Cochliobolus heterostrophus, and as these tools are in modular BioBrick format, they can be easily used to facilitate genetic engineering of other fungal species. |
| Databáze: | OpenAIRE |
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