The Protein Kinase C Pathway Inhibits Iodide Uptake by Calf Thyroid Cells via Sodium Potassium-Adenosine Triphosphatase
| Autor: | Mario A. Pisarev, D. Silberschmidt, L. Krawiec, Guillermo Juvenal, L. V. Bocanera, G. Nocetti |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
medicine.medical_specialty
Indoles Time Factors Endocrinology Diabetes and Metabolism Sodium ATPase Iodide Thyroid Gland Thyrotropin chemistry.chemical_element Stimulation Maleimides chemistry.chemical_compound Endocrinology Internal medicine medicine Animals Enzyme Inhibitors Receptor Cells Cultured Protein Kinase C Protein kinase C chemistry.chemical_classification Forskolin biology Chemistry Colforsin Iodides Bucladesine Phorbol biology.protein Tetradecanoylphorbol Acetate Cattle Sodium-Potassium-Exchanging ATPase Iodine |
| Zdroj: | Thyroid. 11:813-817 |
| ISSN: | 1557-9077 1050-7256 |
| DOI: | 10.1089/105072501316973055 |
| Popis: | The effect of the phorbol esther phorbol myristate acetate (PMA) on iodide uptake was studied in primary cultures of calf thyroid cells. PMA caused a dose- and time-dependent inhibition of thyrotropin (TSH), forskolin, and db-cAMP stimulation, indicating an effect distal to both TSH receptor and cAMP generation. No action was found on iodide efflux, indicating a selective inhibition of iodide uptake. This inhibition was observed even after 5 minutes of incubation, thus excluding a possible genomic action. Bisindolmaleimide (BS), a specific inhibitor of the protein kinase C (PKC) pathway, reverted the effect of PMA. A similar degree of inhibition of the Na+/K+ adenosine triphosphatase (ATPase) and iodide uptake by PMA was found, thus suggesting a link between both parameters. These results indicate that the PKC pathway inhibits thyroid iodide uptake by an action distal to cAMP generation and probably because of a decrease in Na+/K+-ATPase activity. |
| Databáze: | OpenAIRE |
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