Inhibition of choriocarcinoma by Fe3O4-dextran-anti-ß-human chorionic gonadotropin nanoparticles containing antisense oligodeoxynucleotide of heparanase

Autor: Pan Yifeng, Cai Jingting, Tang Dihong, Xia Man, Liu Huining, Yang Ting, Zhang Yi
Jazyk: angličtina
Rok vydání: 2013
Předmět:
Gene Expression
Pharmaceutical Science
Oligodeoxyribonucleotides
Antisense
heparanase
Human chorionic gonadotropin
Mice
International Journal of Nanomedicine
Drug Discovery
Chorionic Gonadotropin
beta Subunit
Human
Magnetite Nanoparticles
Fe3O4-dextran-anti-βHCG nanopar-ticles
Glucuronidase
Original Research
Drug Carriers
Matrigel Invasion Assay
Antibodies
Monoclonal
Dextrans
General Medicine
Transfection
invasive ability
embryonic structures
Female
Gonadotropin
hormones
hormone substitutes
and hormone antagonists
endocrine system
medicine.drug_class
Biophysics
Mice
Nude
Antineoplastic Agents
Bioengineering
Biology
Biomaterials
Cell Line
Tumor
choriocarcinoma
medicine
Animals
Humans
Heparanase
RNA
Messenger
antisense oligodeoxynucleotide
Cell Proliferation
Cell growth
Organic Chemistry
Choriocarcinoma
Neoplasms
Experimental
medicine.disease
Xenograft Model Antitumor Assays
Molecular biology
Cell culture
Zdroj: International Journal of Nanomedicine
ISSN: 1178-2013
Popis: Liu Huining,1 Zhang Yi,1 Tang Dihong,2 Pan Yifeng,3 Xia Man,2 Yang Ting,2 Cai Jingting1,2 1Department of Obstetrics and Gynecology, Xiangya Hospital, 2Department of Gynecological Oncology, Hunan Provincial Tumor Hospital, 3National Hepatobiliary and Enteric Surgery Research Center, Central South University, Changsha, Hunan, People's Republic of China Objective: To observe the influence of Fe3O4-dextran-anti-ß-human chorionic gonadotropin (HCG) carrying heparanase (Hpa) antisense oligodeoxynucleotide (ASODN), via the invasion, proliferation, and Hpa expression of JEG-3 cell lines and inhibitory effect of transplanted choriocarcinoma tumor growth. Methods: The different abilities of invasion and proliferation between transfected JEG-3 and untransfected JEG-3 were measured by Matrigel invasion assay and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay in vitro. The effect of Hpa ASODN transfection on the expression of Hpa mRNA and protein was measured by reverse-transcription polymerase chain reaction and Western blot. The transplanted choriocarcinoma tumors were taken out to calculate the inhibitory effect on tumor growth of Hpa ASODN. Results: In this study, we found that: (1) the invasive ability of JEG-3 cells was inhibited sufficiently (P < 0.05) after JEG-3 cells were transfected by Fe3O4-dextran-anti-βHCG carrying Hpa ASODN; (2) after JEG-3 cells were transfected by Fe3O4-dextran-anti-βHCG carrying Hpa ASODN at 48 and 72 hours, the proliferative ability of JEG-3 cells was inhibited sufficiently (P < 0.05); (3) the expression of Hpa mRNA and protein in JEG-3 cells was inhibited efficiently after JEG-3 cells were transfected by Fe3O4-dextran-anti-βHCG carrying Hpa ASODN (P < 0.05); and (4) Fe3O4-dextran-anti-βHCG carrying Hpa ASODN had an inhibitory effect on the transplanted choriocarcinoma tumor growth (P < 0.05) and was harmless on nude mice. Conclusion: Fe3O4-dextran-anti-βHCG carrying Hpa ASODN weakened the invasive and proliferative ability of choriocarcinoma, with a significant inhibitory effect on the transplanted choriocarcinoma tumor. Therefore, Fe3O4-dextran-anti-βHCG carrying Hpa ASODN is an effective gene therapy, and Fe3O4-dextran-anti-βHCG nanoparticles are a harmless and effective gene vector. Keywords: heparanase, antisense oligodeoxynucleotide, Fe3O4-dextran-anti-βHCG nanoparticles, choriocarcinoma, invasive ability
Databáze: OpenAIRE
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