Effects of the serine protease inhibitor rBmTI-A in an experimental mouse model of chronic allergic pulmonary inflammation
| Autor: | Mílton de Arruda Martins, Adriana Feliciano Alves Duran, Iolanda F.L.C. Tibério, Fernanda D.T.Q.S. Lopes, Edna A. Leick, Sergio D. Sasaki, Beatriz Mangueira Saraiva-Romanholo, Robson Santos de Almeida, Ariana Correa Florencio, Fernanda Magalhães Arantes-Costa |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
medicine.medical_treatment lcsh:Medicine Pharmacology 0302 clinical medicine lcsh:Science Lung Saline Mice Inbred BALB C Multidisciplinary Inhalation medicine.diagnostic_test biology Respiration Chronic inflammation respiratory system Recombinant Proteins Extracellular Matrix medicine.anatomical_structure Matrix Metalloproteinase 9 Bronchial hyperresponsiveness Matrix Metalloproteinase 1 medicine.symptom Bronchoalveolar Lavage Fluid Serine Proteinase Inhibitors Intraperitoneal injection Inflammation Article 03 medical and health sciences Hypersensitivity medicine Animals Author Correction business.industry lcsh:R Pneumonia medicine.disease Asthma Eosinophils Disease Models Animal Ovalbumin 030104 developmental biology Bronchoalveolar lavage Chronic Disease Proteolysis Respiratory Mechanics biology.protein lcsh:Q business Biomarkers 030217 neurology & neurosurgery |
| Zdroj: | Scientific Reports Scientific Reports, Vol 9, Iss 1, Pp 1-13 (2019) |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-019-48577-4 |
| Popis: | To evaluate whether a recombinant serine protease inhibitor (rBmTI-A) modulates inflammation in an experimental model of chronic allergic lung inflammation. Balb/c mice were divided into four groups: SAL (saline), OVA (sensitized with ovalbumin), SAL + rBmTI-A (control treated with rBmTI-A) and OVA + rBmTI-A (sensitized with ovalbumin and treated with rBmTI-A). The animals received an intraperitoneal injection of saline or ovalbumin, according to the group. The groups received inhalation with saline or ovalbumin and were treated with rBmTI-A or saline by nasal instillation. After 29 days, we evaluated the respiratory mechanics; bronchoalveolar lavage fluid (BALF); cytokines; MMP-9, TIMP-1; eosinophils; collagen and elastic fibre expression in the airways; and the trypsin-like, MMP-1, and MMP-9 lung tissue proteolytic activity. Treatment with rBmTI-A reduced the trypsin-like proteolytic activity, the elastance and resistance maximum response, the polymorphonuclear cells, IL-5, IL-10, IL-13 and IL-17A in the BALF, the expression of IL-5, IL-13, IL-17, CD4+, MMP-9, TIMP-1, eosinophils, collagen and elastic fibres in the airways of the OVA + rBmTI-A group compared to the OVA group (p |
| Databáze: | OpenAIRE |
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