Isolation and culture of rat sensory neurons having distinct sensory modalities
Autor: | Stephani P. Eckert, Abraha Taddese, Edwin W. McCleskey |
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Rok vydání: | 1997 |
Předmět: |
Surface Properties
Population Muscle spindle Cell Culture Techniques Sensory system Cell Separation Biology Injections Intramuscular Rats Sprague-Dawley Masseter muscle stomatognathic system Mesencephalon Centrifugation Density Gradient medicine Animals Neurons Afferent education Cells Cultured Fluorescent Dyes education.field_of_study Masseter Muscle Dissection General Neuroscience Tooth Germ Affinity Labels Anatomy Carbocyanines Sensory neuron Rats Solutions medicine.anatomical_structure Nociception Trigeminal Ganglion Nociceptor Pulp (tooth) Neuroglia Neuroscience |
Zdroj: | Journal of Neuroscience Methods. 77:183-190 |
ISSN: | 0165-0270 |
Popis: | We have recently published papers in which sensory neurons that innervate either the tooth pulp or masseter muscle spindles were labelled in vivo and later identified and studied in primary tissue culture (Taddese et al., 1995; Cook et al., 1997). Here, we provide detailed descriptions of cell labelling and tissue culture methods that we used. The purpose of the preparations is to compare nociceptive and non-nociceptive sensory neurons in vitro. The spindles in mastication muscles are the only muscle afferents whose cell bodies reside in the mesencephalic nucleus (MeN5) of the fifth nerve (Corbin and Harrison, J Neurophysiol, 1940; Cody et al., J Physiol, 1972). Thus, labelling neurons projecting to the masseter muscle and dissecting the MeN5 isolates muscle spindle afferents. Pain is the only conscious sensation elicited by physiological stimulus of tooth pulp (Anderson and Matthews, 1967; Edwall and Olgart, 1977; Ahlquist et al., 1984; Narhi et al., 1994); there may be unconscious sensations that arise from the pulp, but these have never been demonstrated. Thus, tooth pulp afferents represent at least a highly enriched, and possibly a pure, population of nociceptors. In broad outline, the methods of labelling and tissue culture are standard, but we have honed many details in order to obtain practical yields. |
Databáze: | OpenAIRE |
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