Autor: |
Kinnersley, Margie, Schwartz, Katja, Yang, Dong-Dong, Sherlock, Gavin, Rosenzweig, Frank |
Rok vydání: |
2021 |
DOI: |
10.6084/m9.figshare.13719421 |
Popis: |
Additional file 1 Fig. S1: Cell density and residual metabolite concentrations. Fig. S2: Input of de novo mutations. Fig. S3: Most de novo mutations only reach low allele frequencies and experience pervasive clonal interference. (A) Histogram of maximum frequencies; (B) Final vs. maximum frequencies; (C) Venn diagram showing degree of genic parallelism among beneficial mutations. Fig. S4: Isolated clones are representative of the populations from which they are drawn. Fig. S5: MalK/MalT population dynamics. Fig. S6: Mutations in glucosyltransferase opgH occur repeatedly and, collectively, go to high frequency. Fig. S7: opgH has nonsense and missense mutations throughout its length. Fig. S8: Mutations that decrease T1 terminator stability in the macromolecular synthesis operon are expected to affect expression of dnaG (DNA primase) and rpoD (housekeeping σ-factor). Predicted ΔG values for wild-type T1 terminator from E. coli K12 MG1655 and two variants observed in chemostat 1 were determined using unafold.rna.albany.edu /?q=mfold/RNA-Folding-Form. The C➔A mutation at nucleotide 3,209,075 has been previously observed in chemostat-evolved E. coli [34]. |
Databáze: |
OpenAIRE |
Externí odkaz: |
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