Impact of emerging pollutants on pulmonary inflammation in asthmatic rats: ethanol vapors and agglomerated TiO2 nanoparticles

Autor: Alexandra Noël, A. Scarino, Michel Charbonneau, Yves Cloutier, Ginette Truchon, Robert Tardif, P. M. Renzi, Renaud Vincent
Přispěvatelé: Institut Armand Frappier (INRS-IAF), Institut National de la Recherche Scientifique [Québec] (INRS)-Réseau International des Instituts Pasteur (RIIP), Département de santé environnementale et santé au travail, Université de Montréal (UdeM), Notre-Dame Hospital, Centre de Recherche du Centre Hospitalier de l’Université de Montréal (CR CHUM), Centre Hospitalier de l'Université de Montréal (CHUM), Université de Montréal (UdeM)-Université de Montréal (UdeM)-Centre Hospitalier de l'Université de Montréal (CHUM), Université de Montréal (UdeM)-Université de Montréal (UdeM), Institut de recherche Robert-Sauvé en santé et en sécurité du travail (IRSST), Inhalation Toxicology Laboratory, Healthy Environments and Consumer Safety Branch, Health Canada
Rok vydání: 2012
Předmět:
Male
MESH: Asthma
MESH: Air Pollutants
Health
Toxicology and Mutagenesis
Toxicology
MESH: Dose-Response Relationship
Drug
MESH: Aerosols
chemistry.chemical_compound
Rats
Inbred BN
MESH: Animals
Lung
Titanium
MESH: Cytokines
Air Pollutants
Inhalation Exposure
biology
Inhalation
Chemistry
respiratory system
MESH: Rats
Inbred BN
medicine.anatomical_structure
MESH: Titanium
[SDV.TOX]Life Sciences [q-bio]/Toxicology
Cytokines
Female
MESH: Inhalation Exposure
medicine.symptom
Bronchoalveolar Lavage Fluid
MESH: Pneumonia
MESH: Ethanol
MESH: Rats
Ovalbumin
Inflammation
Context (language use)
medicine
Animals
MESH: Lung
Asthma
Pollutant
Aerosols
MESH: Ovalbumin
Ethanol
Dose-Response Relationship
Drug
MESH: Bronchoalveolar Lavage Fluid
technology
industry
and agriculture
Pneumonia
medicine.disease
MESH: Male
Rats
Disease Models
Animal
Immunology
biology.protein
MESH: Volatilization
Nanoparticles
MESH: Disease Models
Animal
Volatilization
MESH: Female
MESH: Nanoparticles
Zdroj: Inhalation Toxicology
Inhalation Toxicology, Taylor & Francis, 2012, 24 (8), pp.528-38. ⟨10.3109/08958378.2012.696741⟩
ISSN: 1091-7691
0895-8378
DOI: 10.3109/08958378.2012.696741⟩
Popis: International audience; CONTEXT: Titanium dioxide nanoparticles (nano-TiO(2)) and ethanol vapors are air contaminants with increasing importance. The presence of a pathological pulmonary condition, such as asthma, may increase lung susceptibility to such contaminants. OBJECTIVE: This study aimed to investigate if exposure to inhaled ethanol vapors or nano-TiO(2) can modulate the rat pulmonary inflammatory response resulting from an allergic asthmatic reaction. MATERIALS AND METHODS: Brown Norway rats were sensitized (sc) and challenged (15 min inhalation, 14 days later) with chicken egg ovalbumin (OVA). Leukocytes were counted in bronchoalveolar lavages (BAL) performed at 6, 24, 36, 48 and 72 h following the challenge and either after ethanol exposures (3000 ppm, 6 h/day, daily) or at 48 h (peak inflammation) for nano-TiO(2) exposures (9.35 mg/m(3) aerosol for 6 and 42 h after the OVA challenge). For the nano-TiO(2) exposures, plasma and BAL cytokines were measured and lung histological analyzes were performed. RESULTS: Exposure to ethanol did not significantly affect BAL leukocytes after OVA challenge. Exposure to nano-TiO(2) significantly decreased BAL leukocytes compared to OVA-challenged controls. Plasma and BAL IL-4, IL-6, and INF-γ levels were also decreased in the nano-TiO(2) group. DISCUSSION: While ethanol vapors do not modify the pulmonary inflammation in rats during an asthmatic response, a surprising protective effect for agglomerated nano-TiO(2) was observed. A putative mechanistic basis involving a decrease in the Th2 response caused by OVA is proposed. CONCLUSION: Allergic pulmonary inflammation is not up-regulated by inhalation of the pollutants ethanol and nano-TiO(2). On the contrary, nano-TiO(2) decreases lung inflammation in asthmatic rats.
Databáze: OpenAIRE
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