High-density fecal Enterococcus faecium colonization in hospitalized patients is associated with the presence of the polyclonal subcluster CC17

Autor: Rafael Cantón, Patricia Ruiz-Garbajosa, Rob J. L. Willems, M. de Regt, T. M. Coque, Marc J. M. Bonten, Hermie J. M. Harmsen, F. Baquero
Přispěvatelé: Microbes in Health and Disease (MHD), Groningen Institute for Gastro Intestinal Genetics and Immunology (3GI)
Jazyk: angličtina
Rok vydání: 2012
Předmět:
Male
Enterococcus faecium
medicine.disease_cause
Feces
Medical microbiology
RNA
Ribosomal
16S
Enterococcus faecalis
Cluster Analysis
Colonization
In Situ Hybridization
Fluorescence
Aged
80 and over
0303 health sciences
General Medicine
Middle Aged
3. Good health
Anti-Bacterial Agents
Hospitalization
RNA
Bacterial
Infectious Diseases
Carrier State
Female
Microbiology (medical)
Adult
medicine.medical_specialty
VANCOMYCIN-RESISTANT ENTEROCOCCUS
AMPICILLIN-RESISTANT
Biology
beta-Lactam Resistance
Microbiology
PROBES
03 medical and health sciences
medicine
Humans
Vancomycin-resistant Enterococcus
Gram-Positive Bacterial Infections
030304 developmental biology
Aged
030306 microbiology
ACQUISITION
16S ribosomal RNA
biology.organism_classification
Bacterial Load
Staining
Gastrointestinal Tract
Molecular Typing
FAECALIS
Ampicillin
Zdroj: European Journal of Clinical Microbiology & Infectious Diseases; Vol 31
European Journal of Clinical Microbiology & Infectious Diseases
European Journal of Clinical Microbiology & Infectious Diseases, 31(4), 519-522. SPRINGER
ISSN: 0934-9723
DOI: 10.1007/s10096-011-1342-7
Popis: Enterococcus faecium belonging to the polyclonal subcluster CC17, with a typical ampicillin-resistant E. faecium (AREfm) phenotype, have become prevalent among nosocomial infections around the world. High-density intestinal AREfm colonization could be one of the factors contributing to the successful spread of these pathogens. We aimed to quantify the enterococcal intestinal colonization densities in stool samples from AREfm-colonized and non-colonized patients using fluorescent in situ hybridization (FISH). Stool samples were collected from AREfm-colonized (n = 8) and non-colonized (n = 8) patients. The relative number of Enterococcus faecalis and E. faecium was determined by FISH using specific 16S rRNA probes, while the total amount of bacterial cells was counted by staining the sample with 4',6-diamidino-2-phenylindole (DAPI). The median bacterial cell numbers in fecal samples, counted by DAPI staining, were 7.7 x 10(9) and 4.8 x 10(9) cells/g for AREfm-colonized and non-colonized patients, respectively (p = 0.34). The E. faecium densities in AREfm-colonized patients, accounting for 0.5-7% of all fecal bacterial cells, exceeded E. faecalis levels by over ten-fold. E. faecium was not detected in non-colonized patients. This study demonstrated high E. faecium cell densities in stool samples from patients colonized with AREfm. Increased cell densities may contribute to host-to-host transmission and environmental contamination, facilitating the spread of AREfm in the hospital setting.
Databáze: OpenAIRE
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