Cytotoxicity and antigenicity of antimicrobial synthesized peptides derived from the beetle Allomyrina dichotoma defensin in mice
| Autor: | Ai Asaoka, Kenji Hikosaka, Takashi Isobe, Hisako Saido-Sakanaka, Hideki Hayashidani, Kikuyasu Nakamura, Yukari Koyama, Manabu Yamada, Yoshikazu Hirota, Minoru Yamakawa, Yutaka Nakai, Maki Motobu, Kameo Shimura, Chung Boo Kang |
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| Rok vydání: | 2006 |
| Předmět: |
Antigenicity
Cell Survival Immunology Antimicrobial peptides Peptide Antibodies Microbiology Defensins Mice medicine Animals Immunology and Allergy Antigens Defensin Cells Cultured Antibacterial agent Pharmacology chemistry.chemical_classification Mice Inbred BALB C biology Cyclic peptide Anti-Bacterial Agents Coleoptera chemistry Hemocyanins Macrophages Peritoneal biology.protein Female Immunization Oligopeptides Keyhole limpet hemocyanin Polymyxin B medicine.drug |
| Zdroj: | International Immunopharmacology. 6:1748-1753 |
| ISSN: | 1567-5769 |
| DOI: | 10.1016/j.intimp.2006.06.007 |
| Popis: | Synthetic peptides, peptides A (Arg-Leu-Tyr-Leu-Arg-Ile-Gly-Arg-Arg-NH(2)) and B (Arg-Leu-Arg-Leu-Arg-Ile-Gly-Arg-Arg-NH(2)), derived from the beetle Allomyrina dichotoma defensin, have antimicrobial activities. Immunotoxicological effect of these peptides was evaluated by cytotoxicity of mouse peritoneal macrophages. In addition, antigenicity of these peptides was studied by evaluating antibody responses in mice immunized with these peptides. The toxicity of peptide A toward mouse peritoneal cells was less than that of polymyxin B, when morphologically evaluated in a cytotoxicity test. Almost all of mice injected intraperitoneally (i.p.) with either peptide A or B at 50-150 mg/kg survived, whereas all mice injected i.p. with polymyxin B at the doses of more than 25 mg/kg died within 24 h. Interestingly, almost all of mice injected intravenously with these peptides at the doses of 10 and 25 mg/kg also survived. Furthermore, mice immunized with these peptides conjugated with keyhole limpet hemocyanin (KLH) showed little or negligible anti-peptide A or B antibody production, although anti-KLH antibody was significantly produced. The results indicated that peptides A and B were less cytotoxic than polymyxin B and also had poor antigenicity to produce specific antibody in mice. |
| Databáze: | OpenAIRE |
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