Interactions of zearalanone, α-zearalanol, β-zearalanol, zearalenone-14-sulfate, and zearalenone-14-glucoside with serum albumin
| Autor: | Zelma Faisal, Sándor Kunsági-Máté, Miklós Poór, Virág Vörös, Eszter Fliszár-Nyúl, Beáta Lemli |
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| Rok vydání: | 2020 |
| Předmět: |
Zearalenone-14-sulfate
Swine Serum albumin Toxicology Microbiology Chromatography Affinity 03 medical and health sciences chemistry.chemical_compound 0404 agricultural biotechnology Fusarium Glucosides Affinity chromatography Species differences Animals Humans Toxicokinetics Zearalanone Mycotoxin Zearalenone 030304 developmental biology 0303 health sciences Chromatography biology Zearalanols Chemistry Albumin 04 agricultural and veterinary sciences Mycotoxins Animal Feed 040401 food science Blood proteins Rats Zearalenone-14-glucoside Spectrometry Fluorescence biology.protein Original Article Cattle Protein Binding Biotechnology |
| Zdroj: | Mycotoxin Research |
| ISSN: | 1867-1632 0178-7888 |
| Popis: | The xenoestrogenic mycotoxin zearalenone is a Fusarium-derived food and feed contaminant. In mammals, the reduced (e.g., zearalanone, α-zearalanol, and β-zearalanol) and conjugated (e.g., zearalenone-14-sulfate) metabolites of zearalenone are formed. Furthermore, filamentous fungi and plants are also able to convert zearalenone to conjugated derivatives, including zearalenone-14-sulfate and zearalenone-14-glucoside, respectively. Serum albumin is the dominant plasma protein in the circulation; it interacts with certain mycotoxins, affecting their toxicokinetics. In a previous investigation, we demonstrated the remarkable species differences regarding the albumin binding of zearalenone and zearalenols. In the current study, the interactions of zearalanone, α-zearalanol, β-zearalanol, zearalenone-14-sulfate, and zearalenone-14-glucoside with human, bovine, porcine, and rat serum albumins were examined, employing fluorescence spectroscopy and affinity chromatography. Zearalanone, zearalanols, and zearalenone-14-sulfate form stable complexes with albumins tested (K = 9.3 × 103 to 8.5 × 105 L/mol), while the albumin binding of zearalenone-14-glucoside seems to be weak. Zearalenone-14-sulfate formed the most stable complexes with albumins examined. Considerable species differences were observed in the albumin binding of zearalenone metabolites, which may have a role in the interspecies differences regarding the toxicity of zearalenone. |
| Databáze: | OpenAIRE |
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