Genome-Wide Duplication of Allotetraploid Brassica napus Produces Novel Characteristics and Extensive Ploidy Variation in Self-Pollinated Progeny
| Autor: | Xuan Luo, Liqin Yin, Yang Jin, Kang Zeming, Zou Qiong, Xianhong Ge, Annaliese S. Mason, Yan Long, Maolin Wang, Yu Li, Shaohong Fu, Tao Lanrong, Liangjun Huang, Wang Jisheng, Zhendong Zhu, Tang Rong |
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| Rok vydání: | 2020 |
| Předmět: |
Plant evolution
Genome instability polyploid evolution fungi mitotic abnormality food and beverages QH426-470 Biology Genome genome instability brassica napus Meiosis Polyploid Evolutionary biology pollen aperture Gene duplication Genetics Ploidy Molecular Biology Genome size Genetics (clinical) allopolyploid |
| Zdroj: | G3: Genes, Genomes, Genetics, Vol 10, Iss 10, Pp 3687-3699 (2020) |
| ISSN: | 2160-1836 |
| DOI: | 10.1534/g3.120.401493 |
| Popis: | Whole genome duplications (WGDs) have played a major role in angiosperm species evolution. Polyploid plants have undergone multiple cycles of ancient WGD events during their evolutionary history. However, little attention has been paid to the additional WGD of the existing allopolyploids. In this study, we explored the influences of additional WGD on the allopolyploid Brassica napus. Compared to tetraploid B. napus, octoploid B. napus (AAAACCCC, 2n = 8x =76) showed significant differences in phenotype, reproductive ability and the ploidy of self-pollinated progeny. Genome duplication also altered a key reproductive organ feature in B. napus, that is, increased the number of pollen apertures. Unlike autopolyploids produced from the diploid Brassica species, the octoploid B. napus produced from allotetraploid B. napus had a relatively stable meiotic process, high pollen viability and moderate fertility under self-pollination conditions, indicating that sub-genomic interactions may be important for the successful establishment of higher-order polyploids. Doubling the genome of B. napus provided us with an opportunity to gain insight into the flexibility of the Brassica genomes. The genome size of self-pollinated progeny of octoploid B. napus varied greatly, and was accompanied by extensive genomic instability, such as aneuploidy, mixed-ploidy and mitotic abnormality. The octoploid B. napus could go through any of genome reduction, equilibrium or expansion in the short-term, thus providing a novel karyotype library for the Brassica genus. Our results reveal the short-term evolutionary consequences of recurrent polyploidization events, and help to deepen our understanding of polyploid plant evolution. |
| Databáze: | OpenAIRE |
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