Isolation and characterization of spontaneously immortalized B-lymphocyte lines from HIV-infected patients with and without non-Hodgkin's Lymphoma

Autor: Ke Zhuang, Wenzhe Ho, Deying Guo, Xi-Qiu Xu, Zhigao Xu, Jianhong Peng, Yong Xiong, Yongxi Zhang, Jun Fang, Hongbin Tang, Jin-Biao Liu, Xien Gui, Hang Liu, Feng-Zhen Meng, Xiaoying Qi, Li Zhou, Li Liu, Di Deng, Fuling Zhou, Liang Shao, Huan Liu, Hengning Ke
Rok vydání: 2019
Předmět:
0301 basic medicine
Male
Cancer Research
Lymphocyte
HIV Infections
Cell Separation
Comorbidity
0302 clinical medicine
Circulating tumor cell
immune system diseases
hemic and lymphatic diseases
Original Research
Cancer Biology
CD20
B-Lymphocytes
biology
Lymphoma
Non-Hodgkin
Middle Aged
lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens
Neoplastic Cells
Circulating
medicine.anatomical_structure
Oncology
030220 oncology & carcinogenesis
Monoclonal
Female
Adult
Adolescent
CD3
B‐lymphocyte
lcsh:RC254-282
Peripheral blood mononuclear cell
NHL
immortalized
03 medical and health sciences
Young Adult
medicine
Animals
Humans
Radiology
Nuclear Medicine and imaging
spontaneous
neoplasms
Aged
Neoplasm Staging
business.industry
medicine.disease
HIV infection
Lymphoma
Non-Hodgkin's lymphoma
030104 developmental biology
Case-Control Studies
biology.protein
Cancer research
business
Neoplasm Transplantation
Zdroj: Cancer Medicine
Cancer Medicine, Vol 8, Iss 15, Pp 6741-6755 (2019)
ISSN: 2045-7634
Popis: Isolation of viable circulating tumor cells (CTC) holds the promise for improving screening, early diagnosis, and personalized treatment of lymphoma. In this study, we isolated and characterized spontaneously immortalized B‐lymphocyte (SIBC) lines from HIV‐infected patients with and without Non‐Hodgkin's Lymphoma (AIDS‐NHL). A total of 22 SIBC lines was isolated from peripheral blood mononuclear cells (PBMC) of HIV‐infected patients with (n = 40) and without (n = 77) clinically detectable NHL, but not from healthy individuals (n = 34). Of these, 8 SIBC lines named HIV‐SIBC were generated from HIV‐infected patients without AIDS‐NHL (10%, 8/77), while 14 SIBCs named AIDS‐NHL‐SIBC were from 13 of the AIDS‐NHL patients (32.5%, 13/40). Among the 14 AIDS‐NHL‐SIBCs, 12 were derived from AIDS‐NHL patients with poor prognoses (survival time less than 1 year). SIBCs displayed markers typical of memory B cells (CD3‐CD20+CD27+) with EBV infection. Moreover, AIDS‐NHL‐SIBCs were representative of CTC as evidenced by monoclonal Ig gene rearrangement, abnormal chromosomal karyotype, and the formation of xenograft tumors, while HIV‐SIBCs generated harbored some features of tumor cells, none had the capacity of xenograft tumor formation, suggesting HIV‐SIBC present the precursor of CTC. These results indicate that SIBCs is associated with poor prognosis in AIDS‐NHL patients and can be isolated from HIV‐infected patients with NHL and without NHL. This findings point to the need for further molecular characterization and functional studies of SIBCs, which may prove the value of SIBCs in the diagnosis, prognoses, and screening for NHL among HIV‐infected patients.
Spontaneously immortalized B‐lymphocyte (SIBC) lines were generated by culturing PBMCs from either HIV‐infected patient with or without AIDS‐NHL. SIBCs are representative of the CTCs and associated with poor prognosis in AIDS‐NHL patients, while SIBCs from HIV‐infected patients without NHL could be a precursor of CTC.
Databáze: OpenAIRE
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