Experimental Hybrid Islet Transplantation
| Autor: | K. Inoue, T. Fujisato, Y. J. Gu, K. Burczak, S. Sumi, M. Kogire, T. Tobe, K Uchida, I Nakai, S. Maetani, Y. Ikada |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
medicine.medical_specialty
geography geography.geographical_feature_category Hepatology business.industry Endocrinology Diabetes and Metabolism Pancreatic islets Insulin medicine.medical_treatment Carbohydrate metabolism medicine.disease Islet Polyvinyl alcohol Transplantation Peritoneal cavity chemistry.chemical_compound Endocrinology medicine.anatomical_structure chemistry Diabetes mellitus Internal medicine Internal Medicine medicine business |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 0885-3177 |
| DOI: | 10.1097/00006676-199209000-00009 |
| Popis: | In this study, we first examined in vitro a polyvinyl alcohol membrane to be used to contain hybrid islet cells, and second we tested a bioartificial pancreas with entrapment of pancreatic islets in polyvinyl alcohol membrane in rats with experimentally induced diabetes. The permeability of the polyvinyl alcohol membrane to different substances was studied in a two-cell chamber system. Glucose, insulin, and nutrients passed through the membrane easily, whereas the passage of immunoglobulin G was completely prevented, indicating that this membrane could be effective in protecting the bioartificial pancreas from immunorejection. Approximately 2,000 islets collected from three Sprague-Dawley rats were enclosed in a mesh-reinforced polyvinyl alcohol tube and transplanted into the peritoneal cavity of six Wistar rats with streptozotocin-induced diabetes. Their nonfasting serum glucose levels were significantly decreased for at least 12 days. Six diabetic rats receiving intraperitoneal transplantation of free islets without the tube showed a slight but significant decrease in nonfasting serum glucose levels for only 3 days. One diabetic rat with transplantation of the bioartificial pancreas had a significant and sustained decrease in nonfasting glucose levels from pretransplanted levels of 440-500 mg/dl to a mean value of 162 +/- 13 mg/dl for over 3 months without immunosuppression. The bioartificial pancreas was then removed, and glucose levels gradually increased to over 500 mg/dl. The results of the present study suggest that a bioartificial pancreas with entrapment of islets in a polyvinyl alcohol membrane could be a promising therapeutic approach to diabetes mellitus. |
| Databáze: | OpenAIRE |
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