Molecular Basis for Environment Sensing by a Nucleoid-Structuring Bacterial Protein Filament
| Autor: | Stefan T. Arold, Jacob M. Remington, Jianing Li, Xiaochuan Zhao, Severin T. Schneebeli |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Salmonella typhimurium
Salinity Strain (chemistry) Chemistry Temperature DNA Molecular Dynamics Simulation Structuring Multiscale modeling Article DNA-Binding Proteins Bacterial protein Protein filament chemistry.chemical_compound Bacterial Proteins Protein Domains Biophysics Nucleoid General Materials Science Amino Acid Sequence Protein Multimerization Physical and Theoretical Chemistry Gene Protein Binding |
| Zdroj: | J Phys Chem Lett |
| ISSN: | 1948-7185 |
| DOI: | 10.1021/acs.jpclett.1c01710 |
| Popis: | The histone-like nucleoid structuring (H-NS) protein controls the expression of hundreds of genes in Gram-positive bacteria through its capability to coat and condense DNA. This mechanism requires the formation of superhelical H-NS protein filaments that are sensitive to temperature and salinity, allowing H-NS to act as an environment sensor. We use multiscale modeling and simulations to obtain detailed insights into the mechanism of H-NS filament’s sensitivity to environmental changes. Through the simulations of the superhelical H-NS filament, we reveal how different environments induce heterogeneity of H-NS monomers. Further, we observe that transient self-association within the H-NS filament creates temperature-inducible strain and might mildly oppose DNA binding. At last, we probe different H-NS–DNA complex architectures and show that complexation enhances the stability of both, DNA and H-NS superhelices. Overall, our results provide unprecedented molecular insights into the environmental sensing and DNA interactions of a prototypical nucleoid-structuring bacterial protein filament. |
| Databáze: | OpenAIRE |
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