Comparison of serological and virological findings from subgroup J avian leukosis virus-infected neoplastic and non-neoplastic flocks in Israel

Autor:	M. Malkinson, Richard L. Witter, Irit Davidson, Aly M. Fadly, Caroline Banet-Noach
Rok vydání:	2004
Předmět:	Fluorescent Antibody Technique Enzyme-Linked Immunosorbent Assay Viremia Biology Antibodies Viral Immunofluorescence Virus law.invention Serology Food Animals Antigen law medicine Animals Israel Antigens Viral Poultry Diseases Polymerase chain reaction DNA Primers General Immunology and Microbiology medicine.diagnostic_test Reverse Transcriptase Polymerase Chain Reaction Fibroblasts medicine.disease Virology Avian Leukosis biology.protein Animal Science and Zoology Flock Antibody Chickens
Zdroj:	Avian Pathology. 33:281-287
ISSN:	1465-3338 0307-9457
DOI:	10.1080/0307945042000203380
Popis:	Blood samples from nine broiler breeder flocks comprising five flocks clinically affected with myeloid leukosis tumours (ML+) and four tumour-free flocks from the same commercial background (ML-) were compared for avian leukosis virus subgroup J (ALV-J) serum antibodies by enzyme-linked immunosorbent assay (ELISA), for antigenemia (group-specific antigen) by antigen-trapping ELISA and for viremia. Group-specific antigen was detected in the sera of 58.1% of ML+ birds and 46.4% of the ML- birds (P=not significant), while 45.5% of ML+ birds and 24.1% of the ML- birds had ALV-J antibodies (P=0.065). In inoculated cell culture, 64.1% of the ML+ sera were viremic compared with 16.7% of the ML- sera (P=0.001). Similar significant differences were found between the two groups of flocks when ALV-J viremia was detected by immunofluorescence using a monoclonal env antibody (P=0.004), and for proviral DNA by polymerase chain reaction using two different sets of env-gene primers, H5-H7 (P=0.001) and R5-F5 (P=0.001). Using the primer pair R5-F5 the product size was approximately 1 kbp, while some heterogeneity in size among isolates was discernable. Our results indicate that a combination of diagnostic tests should be adopted in routine examination of tumour material in order to rule out false-negative findings.
Databáze:	OpenAIRE
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