Control of organelle gene expression by the mitochondrial transcription termination factor mTERF22 in Arabidopsis thaliana plants
| Autor: | Mark Markovits, Laure D. Sultan, Sofia Shevtsov, Oren Ostersetzer-Biran, Lior Faigon, Keren Nevo-Dinur, Michal Zmudjak |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine Arabidopsis lcsh:Medicine Gene Expression Mitochondrion 01 natural sciences Biochemistry Gene Expression Regulation Plant Gene expression lcsh:Science Energy-Producing Organelles Flowering Plants Regulation of gene expression Multidisciplinary biology Messenger RNA Gene Expression Regulation Developmental food and beverages Eukaryota Plants Mitochondrial DNA Cell biology Mitochondria Nucleic acids Phenotypes Basic-Leucine Zipper Transcription Factors Genes Mitochondrial Experimental Organism Systems Seeds Cellular Structures and Organelles Research Article Forms of DNA Arabidopsis Thaliana Brassica Bioenergetics Research and Analysis Methods Mitochondrial Proteins 03 medical and health sciences Oxygen Consumption Model Organisms Plant and Algal Models Genetics Plastid Gene Biology and life sciences Arabidopsis Proteins lcsh:R fungi Organisms Correction Cell Biology DNA biology.organism_classification 030104 developmental biology Seedlings RNA lcsh:Q Organelle biogenesis 010606 plant biology & botany |
| Zdroj: | PLoS ONE PLoS ONE, Vol 13, Iss 7, p e0201631 (2018) |
| ISSN: | 1932-6203 |
| Popis: | Mitochondria are key sites for cellular energy metabolism and are essential to cell survival. As descendants of eubacterial symbionts (specifically α-proteobacteria), mitochondria contain their own genomes (mtDNAs), RNAs and ribosomes. Plants need to coordinate their energy demands during particular growth and developmental stages. The regulation of mtDNA expression is critical for controlling the oxidative phosphorylation capacity in response to physiological or environmental signals. The mitochondrial transcription termination factor (mTERF) family has recently emerged as a central player in mitochondrial gene expression in various eukaryotes. Interestingly, the number of mTERFs has been greatly expanded in the nuclear genomes of plants, with more than 30 members in different angiosperms. The majority of the annotated mTERFs in plants are predicted to be plastid- or mitochondria-localized. These are therefore expected to play important roles in organellar gene expression in angiosperms. Yet, functions have been assigned to only a small fraction of these factors in plants. Here, we report the characterization of mTERF22 (At5g64950) which functions in the regulation of mtDNA transcription in Arabidopsis thaliana. GFP localization assays indicate that mTERF22 resides within the mitochondria. Disruption of mTERF22 function results in reduced mtRNA accumulation and altered organelle biogenesis. Transcriptomic and run-on experiments suggest that the phenotypes of mterf22 mutants are attributable, at least in part, to altered mitochondria transcription, and indicate that mTERF22 affects the expression of numerous mitochondrial genes in Arabidopsis plants. |
| Databáze: | OpenAIRE |
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