Polymerase Chain Reaction with Double Primer Pairs for Detection of Human Parvovirus B19 Induced Aplastic Crises in Family Outbreaks
| Autor: | Britt-Marie Eriksson, Barbro Larsson, Eva Fridell, Albert N. Békássy |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Adult
Male Microbiology (medical) Adolescent Molecular Sequence Data DNA Single-Stranded Erythema Infectiosum Dot blot Spherocytosis Hereditary Antibodies Viral Polymerase Chain Reaction Disease Outbreaks Serology law.invention law Parvovirus B19 Human medicine Humans Aplastic anemia Child Polymerase chain reaction Family Health Parvoviridae Base Sequence General Immunology and Microbiology biology Parvovirus Anemia Aplastic virus diseases General Medicine biology.organism_classification medicine.disease Virology Molecular biology Infectious Diseases Child Preschool DNA Viral Female Primer (molecular biology) Nested polymerase chain reaction |
| Zdroj: | Scandinavian Journal of Infectious Diseases. 24:275-282 |
| ISSN: | 1651-1980 0036-5548 |
| Popis: | Parvovirus B19 DNA can be detected by polymerase chain reaction with double primer pairs (nested PCR). Recent infection was documented by a retrospective serological study using Parvoscan-B19 enzyme linked immunosorbent assay (EIA) for detection of B19 human parvovirus IgM and IgG antibodies in serum or plasma specimens. In 3 families B19 outbreaks caused aplastic crises necessitating blood transfusion in 5 children and 1 adult with hereditary sphaerocytosis. Four members from 2 of the families had clinically overt haemolytic anaemia prior to the event. Two members in another family presented with an aplastic crisis disclosing the underlying chronic haemolytic disease. All 7 patients were identified as PCR positive in serum samples taken 3-14 days after the onset of symptoms. Comparison with dot blot hybridization revealed detectable DNA in only 2/3 PCR positive patients. Thus, nested PCR is more sensitive than the dot blot hybridization method and is therefore a suitable complement to the antibody assay for identifying recent B19 infection. |
| Databáze: | OpenAIRE |
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