Noninvasive indirect imaging of vascular endothelial growth factor gene expression using bioluminescence imaging in living transgenic mice
| Autor: | Michael Carey, Alexander J. Annala, Sanjiv S. Gambhir, Lily Wu, Meera Iyer, Yanling Wang |
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| Rok vydání: | 2006 |
| Předmět: |
Transcriptional Activation
Vascular Endothelial Growth Factor A Luminescence Transcription Genetic Physiology Mice Transgenic Biology Mice chemistry.chemical_compound Luciferases Firefly Gene expression Genetics Animals Humans Bioluminescence imaging Whole Body Imaging Regulation of gene expression Wound Healing Mammary tumor Reporter gene Gene Expression Profiling Cobalt Molecular biology Cell Hypoxia Up-Regulation Vascular endothelial growth factor Disease Models Animal Vascular endothelial growth factor A Gene Expression Regulation chemistry Tumor progression Cancer research HeLa Cells |
| Zdroj: | Physiological Genomics. 24:173-180 |
| ISSN: | 1531-2267 1094-8341 |
| Popis: | Vascular endothelial growth factor (VEGF) plays a critical role in the early activation of stromal tissues during wound healing and tumor growth. We report the use of a two-step transcriptional amplification (TSTA) approach to augment the transcriptional activity of the relatively weak VEGF promoter (pVEGF) using firefly luciferase ( fl) reporter gene and bioluminescence imaging (BLI). In cell culture, we demonstrate that TSTA-based fl gene expression can be significantly enhanced over the direct one-step system. Using a transgenic mouse model (pVEGF-TSTA- fl), we demonstrate the induction of VEGF gene expression using a wound-healing model and a subcutaneous mammary tumor model. In skin-wounding experiments, pVEGF-induced fl expression in the wound lesion is detected on days 4 and 5 and peaks on days 15–22. Furthermore, the bioluminescence signal shows good correlation with the endogenous VEGF protein levels in the wound tissue ( r2= 0.70). In the mammary tumor model, fl expression is detected on day 3, peaks at day 17, and declines thereafter. These results support the use of noninvasive BLI for the longitudinal monitoring of VEGF induction during wound healing and tumor progression, and this mouse model should find use in various applications in which it is important to noninvasively study VEGF gene expression. |
| Databáze: | OpenAIRE |
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