Diethylamino hydroxybenzoyl hexyl benzoate (DHHB) as additive to the UV filter avobenzone in cosmetic sunscreen formulations: evaluation of the photochemical behavior and photostabilizing effect
| Autor: | Camila Martins Kawakami, Bianca Bueno Fontanezi, Leandro N.C. Máximo, Lorena Rigo Gaspar, Roberto Santana da Silva |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
030103 biophysics Ultraviolet Rays Chemistry Pharmaceutical Pharmaceutical Science UV filter 02 engineering and technology Photochemistry Aminophenols Fluorescence spectroscopy Cell Line FILTRO SOLAR 03 medical and health sciences chemistry.chemical_compound Benzophenones Mice Drug Stability Spectrophotometry medicine Animals Photodegradation Mice Inbred BALB C Propiophenones Quenching (fluorescence) medicine.diagnostic_test 3T3 Cells 021001 nanoscience & nanotechnology Diethylamino hydroxybenzoyl hexyl benzoate chemistry Avobenzone 0210 nano-technology Phototoxicity Sunscreening Agents |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| Popis: | The aim of the present study was to investigate the photochemical behavior of DHHB and its photostabilizing effect on avobenzone (AVO) in different sunscreen formulations. The formulations were subjected to photostability studies by HPLC and spectrophotometry. In vitro phototoxicity was assessed using 3T3 fibroblast cultures. The mechanism of interaction between DHHB and AVO was investigated by steady state and time-resolved fluorescence spectroscopy. All formulations provided ultra-protection against UVA radiation. HPLC results demonstrated that DHHB did not present a photostabilizing effect on AVO. Fluorescence spectroscopy showed that AVO and DHHB interact by a static quenching mechanism and DHHB did not affect the AVO excited state lifetime. In addition, the energy transfer by Forster mechanism (FRET), which is the most often mechanism responsible for singlet-singlet quenching, is unlikely in this work. These results suggest why DHHB did not work as a photostabilizer on AVO singlet excited state. Phototoxicity results demonstrated that combinations containing DHHB (C2) did not show a phototoxic potential. Finally, although DHHB was considered to be photostable for all formulations studied (F2 and F3) it did not increase the photostability of AVO (F3). Thus, we suggested that formulations containing DHHB (F2) should be considered more advantageous than formulations containing AVO and AVO/DHHB (F1 and F3 respectively). |
| Databáze: | OpenAIRE |
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