A Genome-Wide Screen Identifies Genes That Affect Somatic Homolog Pairing inDrosophila
Autor: | Justine E. Johnson, Barbara G. Mellone, Jack R. Bateman, Kyle E. Dempsey, Mitzi I. Kuroda, Erica Larschan, Lauren S. Marshall, Ryan S D'Souza |
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Rok vydání: | 2012 |
Předmět: |
Male
Candidate gene X Chromosome Somatic cell Investigations Biology medicine.disease_cause Genome 03 medical and health sciences 0302 clinical medicine RNA interference Genetics medicine Animals Drosophila Proteins Guanine Nucleotide Exchange Factors Crossing Over Genetic male-specific lethal Molecular Biology Gene Alleles In Situ Hybridization Fluorescence Genetics (clinical) RNA Double-Stranded 030304 developmental biology 0303 health sciences Mutation Gene knockdown Histone-Lysine N-Methyltransferase interchromosomal interaction Chromosome Pairing RNAi Pairing homolog pairing Drosophila RNA Interference cell cycle 030217 neurology & neurosurgery Genome-Wide Association Study |
Zdroj: | G3: Genes|Genomes|Genetics |
ISSN: | 2160-1836 |
DOI: | 10.1534/g3.112.002840 |
Popis: | In Drosophila and other Dipterans, homologous chromosomes are in close contact in virtually all nuclei, a phenomenon known as somatic homolog pairing. Although homolog pairing has been recognized for over a century, relatively little is known about its regulation. We performed a genome-wide RNAi-based screen that monitored the X-specific localization of the male-specific lethal (MSL) complex, and we identified 59 candidate genes whose knockdown via RNAi causes a change in the pattern of MSL staining that is consistent with a disruption of X-chromosomal homolog pairing. Using DNA fluorescent in situ hybridization (FISH), we confirmed that knockdown of 17 of these genes has a dramatic effect on pairing of the 359 bp repeat at the base of the X. Furthermore, dsRNAs targeting Pr-set7, which encodes an H4K20 methyltransferase, cause a modest disruption in somatic homolog pairing. Consistent with our results in cultured cells, a classical mutation in one of the strongest candidate genes, pebble (pbl), causes a decrease in somatic homolog pairing in developing embryos. Interestingly, many of the genes identified by our screen have known roles in diverse cell-cycle events, suggesting an important link between somatic homolog pairing and the choreography of chromosomes during the cell cycle. |
Databáze: | OpenAIRE |
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